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Plasmid#156432PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF, rtta3G, TetO3G (Ctcf Mouse)
UseMouse TargetingExpressionMammalianMutationnoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-tdTHC
Plasmid#112621Purposeexpression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2DepositorInsertsUseCre/LoxTagstdTomatoExpressionMammalianPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS2002
Plasmid#239832PurposeEngineered OrufIscB (NovaIscB) omegaRNA mammalian expressionDepositorInsertNovaIscB omegaRNA scaffold
ExpressionMammalianMutationReplacement of A12-A57 with GAAA in omegaRNA scaf…Promoterhuman U6Available sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ezh2FL
Plasmid#235575PurposeDox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEzh2 (Ezh2 Mouse)
UseCRISPRAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-GRAB_g5-HT3.0
Plasmid#208726PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterEF1aAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
OMM-V5-LOV-Turbo
Plasmid#199665Purposeexpresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsAKAP1 transmembrane domain and V5ExpressionMammalianPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OT1.0
Plasmid#185384PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based oxytocin sensor GRAB_OT1.0
ExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-C-prM-E-NS1
Plasmid#175277PurposeAAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17DDepositorAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2xFKBP-mCh-KDEL
TagsmCherryExpressionMammalianPromoterChicken beta actin (CAG)Available sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-3XFLAG-AGO2
Plasmid#136687PurposeExpresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)DepositorAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Gal:mCherry-uTEV3
Plasmid#135453PurposeYeast expression of uTEV3 fused to mCherryDepositorInsertmCherry-uTEv3
TagsmCherryExpressionYeastMutationS219V mutation improves stability , I138T/S153N/T…PromoterGalAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-fFuCas9-HTBNLS-hph
Plasmid#121092PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 systemDepositorInsertCas9 and hygromycin resist gene
UseCRISPRExpressionBacterialPromotergpdA;trpCAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOtop1_pcDNA3
Plasmid#114677PurposeThis construct was used to express mouse Otop1 in HEK-293 cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOtopetrin 1 (Otop1 Mouse)
ExpressionMammalianMutationSNP (rs49325632): Glycine 396 to AlaninePromoterCMVAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1586
Plasmid#121057PurposemKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertmKate2-T^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized mKate2, and au…ExpressionWormAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE
Plasmid#123362PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.DepositorInsertFirefly luciferase
UseCRISPR, Lentiviral, and LuciferaseExpressionMammalianPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-DIO-SYP-HRP
Plasmid#117186PurposePlasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-eGFP-B-actin
Plasmid#117814PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-BE3-FNLS(RA)
Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3-FNLS
Tags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CMV-NLS-dCas9-NLS
Plasmid#99908PurposeFor the expression of NLS-dCas9-NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO
Plasmid#73471PurposeExpresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulationDepositorInsertmCherry-P2A-FlpO
UseNeurotropic virusAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by