We narrowed to 7,531 results for: Ski

1. CD58-CD4d3+4-bio

   Plasmid

   #73113

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD58 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD58 (CD58 Human)
   

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pHS1516

   Plasmid

   #205979

   Purpose

   FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas7 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Yes-SH2

   Plasmid

   #46532

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Yes (YES1 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 152-261

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-mHopX

   Plasmid

   #16425

   Depositor

   Jonathan Epstein

   Article

   Chen et al Cell. 2002 Sep 20. 110(6):713-23.

   Insert

   Hopx (hop Mouse)
   

   Use

   In situ template

   Mutation

   None

   Available Since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDule-IBBN (G2)

   Plasmid

   #85500

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

   Plasmid

   #217709

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL4 (ADGRL4 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-406

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-51b(+)-Caprola_14

   Plasmid

   #194675

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_14
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+)-Caprola_15

   Plasmid

   #194676

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_15
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

   Plasmid

   #40806

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(48-55) + S222D + S226D

   Promoter

   MSV-LTR

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pGEX Txk-SH2

    Plasmid

    #46526

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Txk (Txk Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 138-258

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer10-UBC9-sh4

    Plasmid

    #168988

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

    Plasmid

    #217682

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB2 (BAI2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-911

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Expression

    Bacterial

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Sh3bp2-SH2

    Plasmid

    #46484

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sh3bp2 (SH3BP2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 450-561

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

    Plasmid

    #130709

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-R-iLACCO1.1

    Plasmid

    #208020

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

    Plasmid

    #194688

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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