We narrowed to 6,468 results for: poly

1. Frt-hspB10

   Plasmid

   #63101

   Purpose

   Expression of HSPB10 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb10 (ODF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPSP6+t-gfp

   Plasmid

   #48130

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6P1-Ataxin3Q22-C14A

   Plasmid

   #22113

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C14A

   Promoter

   Available since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

4. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Use

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB-Haunt E1-PuroT2AmCherry-4A KI

   Plasmid

   #92164

   Purpose

   For lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockin

   Depositor

   Xiaohua Shen

   Article

   Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

   Insert

   Haunt P-Inr2-cDNA KI homology arms
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF11-SFPQ(214-598)

   Plasmid

   #135440

   Purpose

   Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIS1 DICER1 long-mut-let-7

   Plasmid

   #21651

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant let-7 microRNA binding sites

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDF11-SFPQ(276-535)

   Plasmid

   #135436

   Purpose

   Expresses SFPQ 276-535 with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIS1 DICER1 long-mut-all

    Plasmid

    #21653

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutated let-7 and miR103/107 microRNA binding sit…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIS1 DICER1 long-mut-miR103/107

    Plasmid

    #21652

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant miR103/107 microRNA binding sites

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLJM1-SLC35B2-FLAG

    Plasmid

    #154863

    Purpose

    Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ctcctggtgcagtacttc => ttattagtccaatatttt

    Promoter

    CMV

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. R4pGWB401+Citrine

    Plasmid

    #128432

    Purpose

    Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Citrine, yellow fluorescence protein

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIS1 IMP-1 long-mut-let-7

    Plasmid

    #21642

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutant let-7 microRNA binding sites

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET30a-ASXL1-HARE-HTH domain

    Plasmid

    #107164

    Purpose

    Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    ASXL1 (Asxl1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Contains amino acids 2-83

    Promoter

    T7

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Frt-V5-hspB10

    Plasmid

    #63111

    Purpose

    Expression of HSPB10 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-6P1-Ataxin3Q22-UIM*

    Plasmid

    #22114

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    UIM 1, 2, 3 SA-DG

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pPK1E-1-gfp

    Plasmid

    #48133

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    VTC4 (native promoter)

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF11-SFPQ(369-598)

    Plasmid

    #135438

    Purpose

    Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge