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pcDNA1-Tat

Plasmid

#138478

Purpose

Expresses HIV Tat for efficient sgRNA packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Tat

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Klf4

Plasmid

#13370

Purpose

Retroviral expression of mouse Klf4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDG462

Plasmid

#100903

Purpose

SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2

Plasmid

#13367

Purpose

Retroviral expression of mouse Sox2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pRS404-PTEF-Ago1

Plasmid

#22313

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

AGO1

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-MCS

Plasmid

#84008

Purpose

doxycycline-inducible lentiviral vector

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

C-terminal V5-tagged BirA

Tags

V5, His

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

Plasmid

#117006

Purpose

Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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