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We narrowed to 16,985 results for: She

Showing: 10101 - 10150 of 16985 results

  1. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  2. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Expression
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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  3. pSSA-DsRed/eGFP (pXK-442)

    Plasmid
    #208827
    Purpose
    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208827-plasmid-map-sequence-id-417251

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  4. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  5. 1114H

    Plasmid
    #200640
    Purpose
    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males
    Depositor
    Omar Akbari
    Article
    Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.
    Insert
    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200640-plasmid-map-sequence-id-405636

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  13. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

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  15. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid
    #203805
    Purpose
    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Llgl1 (Llgl1 Mouse)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203805-plasmid-map-sequence-id-401577

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  16. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid
    #196973
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GFP and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196973-plasmid-map-sequence-id-388919

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  17. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid
    #196976
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GST and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196976-plasmid-map-sequence-id-388922

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  18. Human DsRed-Monomer CASP9 C287A Mutant

    Plasmid
    #198469
    Purpose
    Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells
    Depositor
    Hannah Rabinowich
    Insert
    Human Caspase-9 C287A Mutant (CASP9 Human)
    Use
    Tags
    DsRed-monomer
    Expression
    Mammalian
    Mutation
    Changed Cysteine 287 to Alanine to eliminate prot…
    Promoter
    CMV
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198469-plasmid-map-sequence-id-390261

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  29. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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  30. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

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  31. pEPQD2KN0899

    Plasmid
    #177092
    Purpose
    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177092-plasmid-map-sequence-id-349582

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  32. pEPQD2KN0895

    Plasmid
    #177091
    Purpose
    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177091-plasmid-map-sequence-id-349581

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  33. pEPQDPKN0892

    Plasmid
    #177089
    Purpose
    MoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177089-plasmid-map-sequence-id-349579

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  34. pEPQD1CB0865

    Plasmid
    #177084
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177084-plasmid-map-sequence-id-349545

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  35. pEPQD1CB0802

    Plasmid
    #177083
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    strictosidine synthase (CrSTR) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177083-plasmid-map-sequence-id-349544

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  36. pEPQD1CB0808

    Plasmid
    #177082
    Purpose
    MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    tryptophan decarboxylase (CrTDC) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177082-plasmid-map-sequence-id-349543

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  37. pEPQD1CB0863

    Plasmid
    #177081
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    secologanin synthase (CrSLS1) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177081-plasmid-map-sequence-id-349542

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  38. pEPQD1CB0790

    Plasmid
    #177075
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    iridoid synthase (CrISY) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177075-plasmid-map-sequence-id-349537

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  39. pEPQD1CB0794

    Plasmid
    #177073
    Purpose
    MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    geraniol 8-oxidase (CrG8H) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177073-plasmid-map-sequence-id-349535

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  40. pEPQD1CB0784

    Plasmid
    #177069
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by nos promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    P19 suppressor of gene silencing from Tomato Bushy Stunt Virus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177069-plasmid-map-sequence-id-349531

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  41. pEPQD1CB0911

    Plasmid
    #177067
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177067-plasmid-map-sequence-id-349529

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  42. pEPQD1CB0776

    Plasmid
    #177066
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    strictosidine synthase (CrSTR) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177066-plasmid-map-sequence-id-349526

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  43. pEPQD1CB0774

    Plasmid
    #177064
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    tryptophan decarboxylase (CrTDC) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177064-plasmid-map-sequence-id-349525

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  44. pEPQD1CB0773

    Plasmid
    #177063
    Purpose
    MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    tryptophan decarboxylase (CrTDC) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177063-plasmid-map-sequence-id-349524

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  45. pEPQD1CB0772

    Plasmid
    #177062
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    secologanin synthase (CrSLS1) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177062-plasmid-map-sequence-id-349519

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  46. pEPQD1CB0771

    Plasmid
    #177061
    Purpose
    MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    secologanin synthase (CrSLS1) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177061-plasmid-map-sequence-id-349518

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  47. pEPQD1CB0769

    Plasmid
    #177059
    Purpose
    MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177059-plasmid-map-sequence-id-349515

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  48. pEPQD1CB0767

    Plasmid
    #177057
    Purpose
    MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177057-plasmid-map-sequence-id-349511

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  49. pEPQD1CB0123

    Plasmid
    #177055
    Purpose
    MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177055-plasmid-map-sequence-id-349503

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  50. pEPQD1CB0122

    Plasmid
    #177054
    Purpose
    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177054-plasmid-map-sequence-id-349502

    Enlarge

Showing: 10101 - 10150 of 16985 results