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CamKII-LGI1-Y433A-pHluorin

Plasmid

#185541

Purpose

Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed tyrosine 433 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-R474Q-pHluorin

Plasmid

#185542

Purpose

Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed arginine 474 for glutamine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-T380A-pHluorin

Plasmid

#185543

Purpose

Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed threonine 380 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-E383A-pHluorin

Plasmid

#185544

Purpose

Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed glutamic acid 383 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-C200R-pHluorin

Plasmid

#185545

Purpose

Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed cysteine 383 for arginine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS_LITE_PATCHED

Plasmid

#215585

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS LITE PATCHED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS LITE PATCHED (HOXD4 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209061

Purpose

Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

KDM6A

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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1529_pAAV-CB-FKBP12-BirA-HA

Plasmid

#208200

Purpose

Express the murine FKBP12-BirA-HA fusion protein in AAV vector

Depositor

Susan Hamilton

Article

Lee et al Commun Biol. 2023 Sep 14;6(1):942. doi:

Insert

Fkbp12 (Fkbp1a Mouse)

Use

AAV

Promoter

Chicken beta-actin

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFB-CG-LYCHOS WT-HA-GFP

Plasmid

#199687

Purpose

Expression of LYCHOS WT in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Tags

10xHis, EGFP, HA, HRV 3C, and His

Expression

Insect

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) ∆DEP-HA

Plasmid

#199683

Purpose

Transient expression of LYCHOS (GPR155) ∆DEP-HA

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Mutation

DEP domain deleted

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS (GPR155) Y551A-HA

Plasmid

#199676

Purpose

Transient expression of LYCHOS (GPR155) Y551A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Mutation

Y551 mutated to A

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED WT-FLAG

Plasmid

#199679

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED WT (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) P44A-HA

Plasmid

#199675

Purpose

Transient expression of LYCHOS (GPR155) P44A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Mutation

P44 mutated to A

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lamp1 FLAG

Plasmid

#199750

Purpose

Lentiviral construct to stably express Lamp1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Lamp1 (Lamp1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) E48Q-HA

Plasmid

#199662

Purpose

Transient expression of LYCHOS (GPR155) E48Q mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Mutation

E48 mutated to Q

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) F43I-HA

Plasmid

#199674

Purpose

Transient expression of LYCHOS (GPR155) F43I mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Mutation

F43 mutated to I

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.444Tmut

Plasmid

#190477

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.444G>T

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.1853Awt

Plasmid

#190478

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGG442

Plasmid

#165616

Purpose

Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t

Use

CRISPR

Tags

3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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61427-sgHtt_10

Plasmid

#157861

Purpose

Expresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM system

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Htt (Htt Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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