Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,586 results for: Trac;

Clear keywords
Showing: 4041 - 4060 of 7586 results

  2. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid
    #210820
    Purpose
    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
    Depositor
    Luke Chao
    Article
    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7
    Inserts
    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    Tags
    Myc, 3xFlag and TwinStrep, 10xHis
    Expression
    Mammalian
    Promoter
    CMV and CMV / IRES2
    Available Since
    July 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210820-plasmid-map-sequence-id-417732

    Enlarge

  6. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid
    #140983
    Purpose
    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha11-RLuc8 (GNA11 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140983-plasmid-map-sequence-id-300767

    Enlarge

  8. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid
    #140978
    Purpose
    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaz-Rluc8 (GNAZ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140978-plasmid-map-sequence-id-300661

    Enlarge

  10. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

    Enlarge

  11. pcDNA5/FRT/TO-GAlpha12-RLuc8

    Plasmid
    #140985
    Purpose
    Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha12-RLuc8 (GNA12 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140985-plasmid-map-sequence-id-300781

    Enlarge

  12. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Promoter
    U6 and CMV
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

    Enlarge

  13. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

    Enlarge

  14. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

    Enlarge

  15. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid
    #140975
    Purpose
    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai3-RLuc8 (GNAI3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140975-plasmid-map-sequence-id-300658

    Enlarge

  16. pcDNA5/FRT/TO-GAlpha13-RLuc8

    Plasmid
    #140986
    Purpose
    Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha13-RLuc8 (GNA13 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140986-plasmid-map-sequence-id-301587

    Enlarge

  17. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid
    #140977
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoB-Rluc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140977-plasmid-map-sequence-id-300660

    Enlarge

  18. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

    Enlarge

Showing: 4041 - 4060 of 7586 results