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Plasmid#190549PurposeMammalian Expression of Kv3.3 K+ channel scFV. Derived from hybridoma N375/67.DepositorInsertKv3.3 K+ channel (Mus musculus) recombinant scFV (Kcnc3 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Alpha4 scFv [N398A/34]
Plasmid#190551PurposeMammalian Expression of GABA(A)R, Alpha4 scFV. Derived from hybridoma N398A/34.DepositorInsertGABA(A)R, Alpha4 (Rattus norvegicus) recombinant scFV (Gabra4 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GABA(A)R, Alpha2 scFv [N399/19]
Plasmid#190552PurposeMammalian Expression of GABA(A)R, Alpha2 scFV. Derived from hybridoma N399/19.DepositorInsertGABA(A)R, Alpha2 (Rattus norvegicus) recombinant scFV (Gabra2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTEVDNIb
Plasmid#122530PurposeBinary plasmid with a TEV (NIb deleted) cloneDepositorInsertTobacco etch virus (NIb deleted) cDNA
UsePotyvirus vectors to produce carotenoids in plantsTagsExpressionMutationPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTV1
Plasmid#122528PurposeIntermediate plasmid 1 to build tobacco etch virus recombinant clonesDepositorInsertTobacco etch virus cDNA fragment
UsePotyvirus vectors to produce carotenoids in plantsTagsExpressionMutationPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTV3
Plasmid#122529PurposeIntermediate plasmid 3 to build tobacco etch virus recombinant cloneDepositorInsertTobacco etch virus cDNA fragment 3
UsePotyvirus vectors to produce carotenoids in plantsTagsExpressionMutationPromoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human-Dysferlin-Original
Plasmid#60216PurposeHuman dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.DepositorInsertDysferlin (DYSF Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPKm-102
Plasmid#90493PurposepcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)DepositorInsertmOrange
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAT105
Plasmid#180510PurposePlasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX with R3 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL004
Plasmid#180608PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertionDepositorInsertPlmCasX with DpbCasX R1 loop
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT526
Plasmid#180513PurposePlasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv1
PlmCasX-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT079
Plasmid#180509PurposePlasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT077
Plasmid#180511PurposePlasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zAsCpf1
Plasmid#107185Purposeplasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)DepositorInsertAsCpf1
UseCRISPRTagsNLS-3xHAExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-hLbCpf1
Plasmid#107187Purposeplasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)DepositorInserthLbCpf1
UseCRISPRTagsNLS-3xHAExpressionMutationPromotersp6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW1385
Plasmid#229879PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
UseTagsmScarlet-I-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1391
Plasmid#229877PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Psnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPsnt-1::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
UseTagsmScarlet-I-C1ExpressionWormMutationPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAT100
Plasmid#180512PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only