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Plasmid#232410PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC83_HBB(HBBreg-HBA1)
Plasmid#232409PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX313_PELO_WT
Plasmid#228938PurposeConstitutive expression of wild-type human PELODepositorInsertPELO (PELO Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Glu
Plasmid#218458PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Glu substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationThr1273Glu substitutionPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-His1271Lys
Plasmid#218456PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (His1271Lys substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationHis1271Lys substitutionPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-SARS-CoV-2-S-tail-Lys1269Ala-His1271Ala
Plasmid#218459PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Lys1269Ala and His1271Ala substitutions) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationLys1269Ala and His1271Ala substitutionsPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051d_sgCiPELO #2
Plasmid#229018PurposeExpression of CRISPRi PELO sgRNA #2DepositorInsertPELO (PELO Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD051d_sgCiPELO #1
Plasmid#229017PurposeExpression of CRISPRi PELO sgRNA #1DepositorInsertPELO (PELO Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST
Plasmid#221118PurposeFAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3-deltaPEST
UseTagsExpressionYeastMutationSTE3 mutant that lacks the PEST domain in the C-t…PromoterSTE3Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-Embigin
Plasmid#221417PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertEmbigin (EMB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu
Plasmid#218454PurposeBacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions (S SARS-CoV-2)
UseTagseXactExpressionBacterialMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp
Plasmid#218457PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationThr1273Asp substitutionPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV PE2
Plasmid#206276PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertPE2
UseCRISPR; Recombinant baculovirus production, multi…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78_R41A
Plasmid#193368PurposeExpression of C9ORF78 R41A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78_R41A (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationR41A, the mutation is located in the binding inte…PromoterT7Available sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK-CMV-mpx
Plasmid#191826Purposempx probe generation for in situDepositorInsertmpx cDNA (mpx Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC2A13_STOP
Plasmid#161316PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC2A13 (SLC2A13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC35F1_STOP
Plasmid#161299PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC35F1 (SLC35F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits