We narrowed to 4,650 results for: HRE
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Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mArf1(T31N)-HA
Plasmid#79411PurposeExpresses C-terminally HA-tagged Arf1(T31N) in mammalian cellsDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-3HA
Plasmid#67616PurposeA pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…Tagsthree inframe 3' HA tagsExpressionMammalianPromoterCMV and SP6Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
ExpressionMammalianMutationsingle amino acid substitution mutant, threonine …Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
Plasmid#159859PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KR
Plasmid#66166PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by arginine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KA
Plasmid#66164PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by alanine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KQ
Plasmid#66165PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-Cdc42(N17)
Plasmid#11400DepositorInsertcell division cycle 42 (CDC42 Human)
TagsYFPExpressionMammalianMutationObtained with Threonine 17 changed to Asparagine.Available SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
Available SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)MutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
TagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
Plasmid#115487PurposeCRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac1(N17)
Plasmid#11395DepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
TagsYFPExpressionMammalianMutationObtained with Threonine 17 mutated to Asparagine.Available SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RQ/TG
Plasmid#25369DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationThreonine 1343 mutated to Glycine Arginine 139…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239D
Plasmid#22220DepositorInsertSnk/Plk2 (PLK2 Human)
ExpressionMammalianMutationsingle amino acid substitution mutant, threonine …Available SinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only