We narrowed to 63,680 results for: cloning

Showing: 4061 - 4080 of 63680 results

Results per page:

Sort results by:

pCDH-TfR-mApple

Plasmid

#179384

Purpose

Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

Depositor

Angus Johnston

Publication

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

Transferrin receptor 1 - mApple (TFRC Human, Synthetic)

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

Plasmid

#188902

Purpose

dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-KRAB(Kox1)-MeCP2

Use

Lentiviral

Tags

NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

Promoter

SFFV

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Publication

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinker

Depositor

Jose-Antonio Daros

Publication

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Expression

Plant

Promoter

35S CaMV

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Publication

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
EF1a-mEmerald-CAPRIN1

Plasmid

#164218

Purpose

Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

Depositor

Jennifer Lippincott-Schwartz

Publication

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF-1a

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

Plasmid

#164611

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

Depositor

Yulong Li

Publication

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Endocannabinoid sensor GRAB_eCB2.0

Expression

Mammalian

Mutation

S383T

Promoter

CMV

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
CMV-LAMP1-HaloTag

Plasmid

#164209

Purpose

Expresses HaloTag tagged LAMP1 under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Publication

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
8xNFAT-ZsG-mCD8

Plasmid

#162747

Purpose

NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pdCas9- AsiA_m2.1

Plasmid

#158065

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

Depositor

Harris Wang

Publication

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m2.1

Expression

Bacterial

Promoter

TetO

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pAAV CMV GCGR-V5-sTurboID (N)

Plasmid

#153009

Purpose

Expresses split-TuboID N-terminal fragment fused to GCGR

Depositor

Alice Ting

Publication

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

GCGR-sTurboID (N) (L73)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_4xMma PylT_FLAG-Mma PylRS AF

Plasmid

#140023

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

Y271A, Y349F

Promoter

EF1

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
TetOn-REEP5-mCherry-eGFP

Plasmid

#139870

Purpose

Dox-inducible lentiviral expression of human REEP5

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

REEP5 (REEP5 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TetOn-CLIMP63-mCherry-eGFP

Plasmid

#139871

Purpose

Dox-inducible lentiviral expression of human CLIMP63

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CLIMP63 (CKAP4 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pLENTI_TetOn_Hu_BIRC5(WT)

Plasmid

#136348

Purpose

Lentiviral inducible expression (TetOn) of human BIRC5

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pHAGE-mt-mKeima-P2A-FRB-Fis1

Plasmid

#135295

Purpose

stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

Depositor

Richard Youle

Publication

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pCAG-Positron

Plasmid

#129253

Purpose

Express Positron in mammalian cells

Depositor

Eric Schreiter

Publication

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Positron

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOX4

Plasmid

#127449

Purpose

Encodes YhaV toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

yhaV toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge