We narrowed to 4,495 results for: Ado

1. pPHR-mCherry-hNCL

   Plasmid

   #181886

   Purpose

   Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-hNCL (NCL Human, Mustard Weed)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-mEmerald-CAPRIN1

   Plasmid

   #164218

   Purpose

   Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   CAPRIN1 (CAPRIN1 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOBI-FLuc

   Plasmid

   #170674

   Purpose

   Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   luc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Human, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ189

   Plasmid

   #162666

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-EF1a ANXA11-mEmerald

   Plasmid

   #164210

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLEX-PGK-LAMP1-HaloTag

   Plasmid

   #164216

   Purpose

   pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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