Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 17,815 results for: Por

Showing: 8161 - 8200 of 17815 results

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pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

Plasmid

#192317

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-EGFR-FRB-GluGlu neo

Plasmid

#192293

Purpose

Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-EGFR-FRB-GluGlu (EGFR Human)

Use

Retroviral

Tags

GluGlu

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-Erbb2-2xFKBP-HA hygro

Plasmid

#192291

Purpose

Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1632X

Plasmid

#198122

Purpose

Express hCOL4A5_S1632X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1632X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_Q1016X

Plasmid

#198119

Purpose

Express hCOL4A5_Q1016X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

Q1016X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_R1683X

Plasmid

#198111

Purpose

lentiviral expression of hCOL4A4_R1683Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1683X

Promoter

EF1a

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_C1684X

Plasmid

#198112

Purpose

lentiviral expression of hCOL4A4_C1684X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C1684X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_C29X

Plasmid

#198107

Purpose

lentiviral expression of hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C29X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_R1563X

Plasmid

#198109

Purpose

lentiviral expression of hCOL4A4_R1563Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1563X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_S36X

Plasmid

#198108

Purpose

lentiviral expression of hCOL4A4_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

S36X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc

Plasmid

#198106

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_G5X

Plasmid

#198114

Purpose

Express hCOL4A5_G5X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

G5X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_C29X

Plasmid

#198115

Purpose

Express hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

C29X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_E130X

Plasmid

#198117

Purpose

Express hCOL4A5_E130X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

E130X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_S36X

Plasmid

#198116

Purpose

Express hCOL4A5_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S36X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_hCOL4A5-Nluc_S1661X

Plasmid

#198123

Purpose

Express hCOL4A5_S1661X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

S1661X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iFluoxSnFR_cyto

Plasmid

#182817

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_ER

Plasmid

#182821

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_PM

Plasmid

#182822

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV9-hSyn-iFluoxSnFR_cyto

Plasmid

#182823

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Use

AAV

Promoter

hSyn

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9-EF-5Usp5

Plasmid

#174873

Purpose

CRISPR vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Usp5 (Usp5 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::UMSBP::sNluc2_ORF2

Plasmid

#186766

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1043

Plasmid

#169621

Purpose

Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2

Expression

Mammalian

Promoter

PhCMV / PPGK

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1042

Plasmid

#169620

Purpose

Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2

Expression

Mammalian

Promoter

PhCMV / PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGB-As-NF-kB1

Plasmid

#180209

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB1 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

GAL4 (1-147) fusion

Expression

Yeast

Mutation

aa 2-409

Promoter

AdhI

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-FLAG-As-NF-kB1

Plasmid

#180200

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKT2/CAGXSP/PalmGamillus

Plasmid

#182971

Purpose

Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmGamillus

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-MYC-As-NF-kB2

Plasmid

#180204

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-MYC-As-NF-kB1

Plasmid

#180203

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB1 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB1

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-409

Promoter

CMV

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGB-As-NF-kB2

Plasmid

#180210

Purpose

Expresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeast

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

GAL4 (aa 1-147) fusion protein

Expression

Yeast

Mutation

aa 2-502

Promoter

AdhI

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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