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DVK_AF

Plasmid

#66068

Purpose

MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDGB1_omega2

Plasmid

#68235

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LI E-F 35S term c1

Plasmid

#54442

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Terminator of Cauliflower mosaic virus CaMV

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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FR_DsRed2

Plasmid

#31444

Depositor

Gerhart Ryffel

Publication

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

DsRed2

Use

Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Sgf73_Sgf11/PCDFDuet

Plasmid

#26964

Depositor

Cynthia Wolberger

Publication

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

sgf11 (SGF11, SGF73 Budding Yeast)

Expression

Bacterial

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28-SpyCatcher002-MBP

Plasmid

#216295

Purpose

Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher002-MBP

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-N3-hARL1(WT)

Plasmid

#206406

Purpose

Mammalian expression of hARL1(WT)

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Publication

Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

Insert

ARL1 (ARL1 Human)

Tags

EGFP

Expression

Mammalian

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_122

Plasmid

#208095

Purpose

Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

Depositor

John Doench

Publication

Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EF1a

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_HDLBP

Plasmid

#154448

Purpose

For recombination of HDLBP

Depositor

Markus Landthaler

Publication

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Expression

Mammalian

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Promoter

U6

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG037

Plasmid

#113995

Purpose

SC module. Super-folder GFP green fluorescence reporter

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Super-folder GFP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac211-APL2-iGFPx6

Plasmid

#105251

Purpose

Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

APL2 portion (APL2 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-mCherry2Bx6

Plasmid

#105253

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pnmr-1

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pcol-19

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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Reb_83 aka PAS624

Plasmid

#71229

Purpose

Reb_1 with RebA(G53A, D89G)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, D89G)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pDGB3_omega1

Plasmid

#68238

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDGB3_alpha2

Plasmid

#68229

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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H2AFY2

Plasmid

#39052

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

H2AFY2 (MACROH2A2 Human)

Tags

His6-Thioredoxin-TEV

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
40 p tie2

Plasmid

#13064

Depositor

Nathan Lawson

Publication

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

tie2 (tek Zebrafish)

Mutation

Q817E (please see depositor's comment below)

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only