We narrowed to 8,835 results for: FIE

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LT3GEPIR-Rb1shRNA2

Plasmid

#220585

Purpose

To inducibly knockdown Rb1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

Rb1 shRNA2 (RB1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-Rb1shRNA3

Plasmid

#220586

Purpose

To inducibly knockdown Rb1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

Rb1 shRNA3 (RB1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

Plasmid

#211819

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and blasticidin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter and miniU6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

Plasmid

#211815

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and puromycin selection gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter, U6 promoter, and hPGK promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

Plasmid

#208399

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

Plasmid

#208398

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

E2-Crimson

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

Plasmid

#208400

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

None

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIFEX-A2aGH

Plasmid

#160542

Purpose

pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX1-A2aGH

Plasmid

#160543

Purpose

pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX2-A2aGH

Plasmid

#160544

Purpose

pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX3-A2aGH

Plasmid

#160545

Purpose

pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX1-A2aGH

Plasmid

#160546

Purpose

pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX2-A2aGH

Plasmid

#160547

Purpose

pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX3-A2aGH

Plasmid

#160548

Purpose

pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p35S_EVDm1

Plasmid

#167121

Purpose

Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE (EVD) (AT5G17125 Mustard Weed)

Expression

Plant

Mutation

mutated four nucleotides at the snoRNA U1 binding…

Promoter

CaMV 35S

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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p35S_GFP-EVDinter-GUS

Plasmid

#167123

Purpose

Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

Depositor

Arturo Marí-Ordóñez, Olivier Voinnet

Article

Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

Insert

EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)

Tags

GUS and mGFP5

Expression

Plant

Promoter

CaMV 35S

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA sCRNA 2.0 (GB2461)

Plasmid

#160593

Purpose

Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA sCRNA 2.0

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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