We narrowed to 20,285 results for: ACE;

1. pTSAR3.1t

   Plasmid

   #210254

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   mCerulean
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAP4

   Plasmid

   #136079

   Purpose

   Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pNAS1B

   Plasmid

   #61968

   Purpose

   Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Serine 65 to Glycine (GGT), Serine 72 to Alanine …

   Promoter

   PBAD and PLtetO

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLPO3

   Plasmid

   #209977

   Purpose

   Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (pAMBeta1)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pET20b-NLuc-MBP-6xHis

   Plasmid

   #135931

   Purpose

   Expresses NanoLuciferase in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDestattB_ubi:EGFP (pCM272)

   Plasmid

   #68339

   Purpose

   Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

   Depositor

   Leonard Zon, Christian Mosimann

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Insert

   EGFP
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   ubi

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mNFAT4-462:TALE:VP64:KRɸ

   Plasmid

   #160662

   Purpose

   Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   mNFAT4-462:TALE:VP64:KRɸ
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pHES830

    Plasmid

    #87944

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn-ConVERGD-N2cG-WPRE

    Plasmid

    #218757

    Purpose

    Provides AND intersectional (Cre+Flp) expression of N2C glycoprotein

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    N2cG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Depositor Comments

    Promoter

    hSyn

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PT7-deGFP-MGapt

    Plasmid

    #67741

    Purpose

    T7 promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T7term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTU2S-a (p15A origin)

    Plasmid

    #74088

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHCKan-yibDp2-GFPuv

    Plasmid

    #139083

    Purpose

    Low phosphate inducible promoter yibDp2 for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    yibDp2-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

    Plasmid

    #160045

    Purpose

    For expressing the active auxin pump PIN2

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    PIN2
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    pEF1s

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge