We narrowed to 9,300 results for: BLI

1. EP004: pAAV TRE::GEM7-HaloTag

   Plasmid

   #209304

   Purpose

   Doxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice

   Depositor

   Adam Cohen

   Insert

   GEM7
   

   Use

   AAV

   Tags

   HaloTag

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAN68

   Plasmid

   #164525

   Purpose

   Retroviral expression of CENP-R uORF-1

   Depositor

   Iain Cheeseman

   Insert

   CENP-R uORF-1 (ITGB3BP Human)
   

   Use

   Retroviral

   Tags

   -6xhis-precission-GFP

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG Retro EGFP Neo

   Plasmid

   #234464

   Purpose

   Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Green Fluorescent Protein
   

   Use

   Retroviral

   Available Since

   March 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCS2-zH2B-GFP

   Plasmid

   #226561

   Purpose

   Express H2B-GFP in zebrafish.

   Depositor

   Yong Zhang

   Insert

   h2b (h2bc1 Zebrafish)
   

   Use

   Synthetic Biology; Zebrafish expression

   Tags

   EGFP

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZP05

   Plasmid

   #232319

   Purpose

   pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum

   Depositor

   Chenggang Wu

   Insert

   Ori-repA
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3'dmDR-CMV-CasRx-bGHpolyA

   Plasmid

   #229556

   Purpose

   Expresses CasRx (RfxCas13d) with double processed direct repeats (15nt) and a non-target spacer in between at 3' end

   Depositor

   Omar Akbari

   Insert

   CasRx
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

   Plasmid

   #221448

   Purpose

   Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3

   Depositor

   Omar Akbari

   Inserts

   wtCas9-NG
   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLaG2-T7RNAPC

   Plasmid

   #216337

   Purpose

   Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP

   Depositor

   Mark Styczynski

   Insert

   LaG2-T7RNAPC fusion
   

   Expression

   Bacterial

   Mutation

   g2658a, g3915a

   Available Since

   Jan. 17, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBS-ACTB-C200-T7

   Plasmid

   #229850

   Purpose

   Donor vector for the ACTB site containing T7 sequence for detection of HR products.

   Depositor

   Natsuko Chiba

   Insert

   Partial sequence of ACTB gene
   

   Use

   Hr donor vector for human actb gene.

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG-scr

    Plasmid

    #229847

    Purpose

    Expresses wild-type Cas9 and scrambled gRNA.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA with scrambled sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid

    #214682

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid

    #214683

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr2
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDual_dsCas9_Venus_hs_TP73

    Plasmid

    #214685

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid

    #214686

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDual_dsCas9_BFP_hs_TP73

    Plasmid

    #214687

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-TRE-miRFP703_PGK-Puro

    Plasmid

    #216667

    Purpose

    Expresses tTa-inducible miRFP703, with puromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    miRFP703
    PuroR
    

    Use

    Lentiviral

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti puro HA-TUBA1B

    Plasmid

    #227587

    Purpose

    Lentiviral plasmid expressing human TUBA1B protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    TUBA1B (TUBA1B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-phSyn-flex-SypHaloTag

    Plasmid

    #172280

    Purpose

    Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

    Depositor

    Danny Winder

    Insert

    synaptophysin-HaloTag
    

    Use

    AAV and Cre/Lox

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    rat synapsin

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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