We narrowed to 4,430 results for: TIL;

1. pAUX_OL

   Plasmid

   #166246

   Purpose

   use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
   

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and BBa_J23116

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9_CL

   Plasmid

   #166245

   Purpose

   regulated dCas9 generator

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   P112-sgRNA-term-P104-RBS1-dCas9-B15
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and Ec-TTL-P104

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reb_206 aka PAS629

   Plasmid

   #71234

   Purpose

   Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJB06

   Plasmid

   #190480

   Purpose

   Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Cas9
   XylR
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Promoter

   xylR-driven promoter

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB07

   Plasmid

   #190481

   Purpose

   Targeting plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Upstream & Downstream pyrE deletion region
   gRNA
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Let-7b-GFP

   Plasmid

   #25404

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   Mirlet7b (Mirlet7b Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-mCherryGGBsMTS

   Plasmid

   #133625

   Purpose

   Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherryGGBsMTS
   

   Expression

   Bacterial

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. XW08

   Plasmid

   #65832

   Purpose

   Punc-4-QF-SL2-mcherry-3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Tags

   mCherry

   Expression

   Worm

   Promoter

   Punc-4

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK1036

    Plasmid

    #17728

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Expression

    Bacterial

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pR184

    Plasmid

    #47952

    Purpose

    rtTA positive feedback loop with three tet operators in the GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6_Ptac_BmHps_BmPhi_BsMdh

    Plasmid

    #129103

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Expression

    Bacterial

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCH001

    Plasmid

    #47953

    Purpose

    tetO-GFP reporter casette

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    GFP
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    T3

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCH043

    Plasmid

    #47949

    Purpose

    rtTA under the control of GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCH044

    Plasmid

    #47950

    Purpose

    rtTA under the control of GAL2 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Expression

    Yeast

    Promoter

    Gal2

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSM002

    Plasmid

    #122936

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML043C (Marked)
    

    Expression

    Yeast

    Mutation

    YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSM001

    Plasmid

    #122935

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML029W (Marked)
    

    Expression

    Yeast

    Mutation

    YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEC005

    Plasmid

    #122939

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML071C (Marked)
    

    Expression

    Yeast

    Mutation

    YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSV_207

    Plasmid

    #122938

    Purpose

    Expression of the marked mRNA under the control of substitutional promoters.

    Depositor

    Attila Becskei

    Article

    Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

    Insert

    YML070W (Marked)
    

    Expression

    Yeast

    Mutation

    YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

    Promoter

    [tetO2]4inPgal1

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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