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We narrowed to 63,680 results for: cloning

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Showing: 4101 - 4120 of 63680 results

  3. pCMV/3' Box_(GLuc)_TOP1

    Plasmid
    #68434
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68434-plasmid-map-sequence-id-120212

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  4. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  5. pMH3

    Plasmid
    #52528
    Purpose
    serves as PCR-template to generate HR donors for C-terminal GFP-tag
    Depositor
    Klaus Foerstemann
    Publication
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP and Blasticigin resistance cassette
    Use
    Blunt cloning kit
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52528-plasmid-map-sequence-id-81488

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  9. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 4101 - 4120 of 63680 results