We narrowed to 13,996 results for: eng
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Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF073 MBP-Axin-His
Plasmid#154011PurposeExpresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opto-casp9_VfAU1-LOV_casp9
Plasmid#131220PurposeExpresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)DepositorInsertOpto-casp9 (VfAU1-LOV_casp9)
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgTP53(R273)
Plasmid#85561PurposeExpresses sgRNA targeting human TP53 around codon R273DepositorInserttumor protein p53 (TP53 Human)
ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SpSrtA
Plasmid#64987PurposeExpresses the wild type of Streptococcus pyogenes sortase A in E. coliDepositorInsertS. pyogenes sortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-81PromoterT7 promoterAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP116
Plasmid#207918PurposeGreenBraid destination vector pGBZ1 - PaqCI - Eco31I - spisPink- Eco31I - PaqCIDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3L
Plasmid#126588PurposeDNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorAvailable SinceDec. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-iRFP-p63DH
Plasmid#176120PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
ExpressionMammalianAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_n-1] (GB1210)
Plasmid#75411PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [M1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianPromoterhU6, CBhAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)
Plasmid#214881PurposeLentiviral vector encoding RfxCas13d targeting B2M guideDepositorInserthU6-crB2M-EFS-PuroR-WPRE
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only