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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

Plasmid

#154029

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pYC01

Plasmid

#224586

Purpose

IPTG-inducible phage φX174 lysis gene E

Depositor

Ophelia Venturelli

Publication

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

phage φX174 lysis gene E (phiX174p08 )

Use

Synthetic Biology

Expression

Bacterial

Promoter

PA1lacO-1

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Publication

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Expression

Bacterial

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTn7xTS-mPlum

Plasmid

#117392

Purpose

Tn7 tagging vector pTn7xTS with mPlum expression scaffold

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTn7xKS-mPlum

Plasmid

#117396

Purpose

Tn7 tagging vector pTn7xKS with mPlum expression scaffold

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Publication

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mPax1 (CT#1039)

Plasmid

#13902

Depositor

Cliff Tabin

Publication

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Pax1 (Pax1 Mouse)

Use

Retroviral; Avian expression

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pBAD-rxRFP1.2

Plasmid

#67839

Purpose

redox-sensitive red fluorescent protein rxRFP1.2

Depositor

Huiwang Ai

Publication

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.2

Tags

His Tag

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pGGD005

Plasmid

#48853

Purpose

Provides linker:BFP as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Linker:BFP

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBY2807

Plasmid

#23222

Depositor

Ralf Baumeister

Publication

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

Gateway-SnAvi

Expression

Bacterial, Insect, and Mamm…

Available since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV2 TEV-ORF3A

Plasmid

#154416

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of o…

Mutation

Many synonymous changes due to codon optimization

Available since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-NSP12

Plasmid

#154410

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available since

July 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-NSP1

Plasmid

#154400

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop

Plasmid

#154030

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQAT56

Plasmid

#223428

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Publication

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Publication

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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