We narrowed to 5,692 results for: org

1. pUltra-hot-H2B-mTagBFP2

   Plasmid

   #161792

   Purpose

   3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-mTagBFP2
   

   Use

   Lentiviral; Multicistronic

   Tags

   mCherry and mTagBFP2

   Expression

   Mammalian

   Mutation

   I174A in the mTagBFP2 protein according to PLoS O…

   Promoter

   UbC

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLRD26

   Plasmid

   #208273

   Purpose

   Production of levopimaradiene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   LPS
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mouse pGL3-ank3_1-promoter firefly luciferase

   Plasmid

   #86436

   Purpose

   Mouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1e

   Depositor

   Hans-Georg Koenig, Jochen Prehn

   Insert

   ank3_1 promoter
   

   Tags

   n/a

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   ankyrin-3_1

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCYP22

   Plasmid

   #212855

   Purpose

   Expresses the Scytonema sp. HK-05 LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Promoter

   PcymRC

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLRD25

   Plasmid

   #208272

   Purpose

   Production of cis-abienol in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   abCAS
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMarC9-R6k

   Plasmid

   #89477

   Purpose

   transposon plasmid, compatible with Tn-Seq

   Depositor

   George Church

   Article

   Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

   Insert

   pMarC9-R6k
   

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

   Plasmid

   #118156

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-H-benM

    Plasmid

    #73925

    Purpose

    Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

    Depositor

    George Church

    Article

    Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

    Insert

    muconate biosensing apparatus
    

    Available Since

    Jan. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDR_DDX4-T2A-tdTomato-puroTK

    Plasmid

    #222519

    Purpose

    Homology directed repair template for knocking in tdTomato reporter to DDX4.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    tdTomato
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330_SYCP3_nterm

    Plasmid

    #222524

    Purpose

    Cas9/sgRNA plasmid for targeting SYCP3

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEV4

    Plasmid

    #110539

    Purpose

    The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

    Depositor

    William B. Whitman

    Article

    Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pRab-3::Cerulean-Venus::Lgg-1

    Plasmid

    #68045

    Purpose

    Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pRab-3::Cerulean-Venus::Lgg-1
    

    Expression

    Worm

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLRD18

    Plasmid

    #208270

    Purpose

    Production of ent-isokaurene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    ptmT2
    osKSL6
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLRD10

    Plasmid

    #208262

    Purpose

    Production of labda-7,13(16),14-triene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    grTPS2
    sclav_p0491
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pStrep-1xEGFP-N1

    Plasmid

    #122488

    Purpose

    Expression of Strep-tagged 1xEGFP

    Depositor

    Georg Mayr

    Article

    Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

    Insert

    Strep-tagged 1xEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEft-3::Cerulean-Venus::Tomm-7

    Plasmid

    #68046

    Purpose

    Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

    Depositor

    Dana Miller

    Article

    Chapin et al Aging (Albany NY). 2015 Jun 30.

    Insert

    pEft-3::Cerulean-Venus::Tomm-7
    

    Expression

    Worm

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid

    #71659

    Purpose

    Mammalian expression of GEMIN6

    Depositor

    Gideon Dreyfuss

    Insert

    GEMIN6 (GEMIN6 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pHDR_REC8-T2A-mGreenLantern-puroTK

    Plasmid

    #222521

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to REC8.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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