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PET.SUMO eIF4G1 +MIC 1-200

Plasmid

#158792

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon) codon optimized

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-puro

Plasmid

#167824

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72L-T2A-GFP

Plasmid

#163784

Purpose

Expression of R72L CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Mutation

R72L

Promoter

MND

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-puro

Plasmid

#167830

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a>RPS2-APOBEC1-p2a-TdTomato-WPRE249-BGHpA

Plasmid

#254839

Purpose

Ribo-STAMP AAV to detect translation dynamics

Depositor

Eugene Yeo

Article

Sison et al Nature. 2026 Feb 18. doi: 10.1038/s41586

Insert

RPS2 (RPS2 Human)

Use

Adenoviral

Tags

APOBEC1-HA-P2A-TdTomato

Expression

Mammalian

Promoter

EF-1 alpha

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_Khalf-Halo

Plasmid

#247485

Purpose

Expresses H1.2 (Khalf) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Half of Lysine in C-terminal IDR are replaced by …

Promoter

Tet-responsive element

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaSKE_ALFA_231232

Plasmid

#250444

Purpose

Expresses tagged alpha skeletal muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Skeletal Muscle Actin (ACTA1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaCARD_ALFA_231232

Plasmid

#250443

Purpose

Expresses tagged alpha cardiac muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Cardiac Muscle Actin (ACTC1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaSMA_ALFA_230231

Plasmid

#250442

Purpose

Expresses tagged gamma smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T230/A231.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Smooth Muscle Actin (ACTG2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaSMA_ALFA_231232

Plasmid

#250441

Purpose

Expresses tagged alpha smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Smooth Muscle Actin (ACTA2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_GammaActin_ALFA_229230

Plasmid

#250440

Purpose

Expresses tagged gamma cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Gamma Cytoplasmic Actin (ACTG1 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMWCAVI_H6BAP-OROV_N

Plasmid

#240162

Purpose

Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

Expression

Bacterial

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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