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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,703 results for: sequence

Showing: 4101 - 4120 of 12703 results

  1. AAV Ef1a-EGFP-WPRE

    Plasmid
    #135428
    Purpose
    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence
    Depositor
    Rylan Larsen
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135428-plasmid-map-sequence-id-271147

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  2. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  3. pT7-MS2-HA-C1-HsYTHDF1_AB

    Plasmid
    #148316
    Purpose
    Mammalian Expression of HsYTHDF1
    Depositor
    Elisa Izaurralde
    Insert
    HsYTHDF1 (YTHDF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148316-plasmid-map-sequence-id-474970

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  4. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Use
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

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  5. NKTR

    Plasmid
    #25597
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NKTR (NKTR Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pT7-V5-SBP-C1-HsUpf1-siRNAres_P

    Plasmid
    #147196
    Purpose
    Mammalian Expression of HsUpf1-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147196-plasmid-map-sequence-id-429667

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  7. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid
    #148120
    Purpose
    Mammalian Expression of HshnRNPH1
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148120-plasmid-map-sequence-id-396934

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  8. pJK406

    Plasmid
    #72420
    Purpose
    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72420-plasmid-map-sequence-id-135533

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  9. Z-MATR3-4

    Plasmid
    #166558
    Purpose
    scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166558-plasmid-map-sequence-id-329266

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  10. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  11. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid
    #147163
    Purpose
    Insect Expression of DmBicaudalC
    Depositor
    Elisa Izaurralde
    Insert
    DmBicaudalC (BicC Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    12 silent and three non silent mutattions I331T, …
    Promoter
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147163-plasmid-map-sequence-id-355687

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  12. pLXIN2-EGFP

    Plasmid
    #99204
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    EGFP
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was inserted 5' to ATG to …
    Promoter
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    99204-plasmid-map-sequence-id-192204

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  13. A-WDR5-1

    Plasmid
    #166515
    Purpose
    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166515-plasmid-map-sequence-id-327811

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  14. p(+)MV-NSE-FlagrP-3g_haP-M502-3p

    Plasmid
    #58798
    Purpose
    Expresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Use
    Tags
    Flag and Ha
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58798-plasmid-map-sequence-id-91093

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  15. pDual_dsCas9_Venus_hs_NQO1

    Plasmid
    #214684
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1
    Depositor
    Kevin Janes
    Insert
    dgRNA_NQO1 (NQO1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214684-plasmid-map-sequence-id-428771

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  16. pET41-FeoB-OL2del

    Plasmid
    #216742
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [LEDSGYMA…
    Promoter
    Available since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216742-plasmid-map-sequence-id-446359

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  17. pET41-FeoB-OL4del

    Plasmid
    #216744
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216744-plasmid-map-sequence-id-434438

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  18. pET41-FeoB-OL5del

    Plasmid
    #216745
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 5 (OL5) with the sequence of [ATFAA] i…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216745-plasmid-map-sequence-id-434437

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  19. pET41-FeoB-OL1del

    Plasmid
    #216741
    Purpose
    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    feoB (PA4358 P. aeruginosa (Bacteria))
    Use
    Tags
    8x His
    Expression
    Bacterial
    Mutation
    Outer loop 1 (OL1) with the sequence of [INIGGALQ…
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216741-plasmid-map-sequence-id-434055

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  20. pAc5.1B-EGFP-DmRigor-mortis_AA

    Plasmid
    #148203
    Purpose
    Insect Expression of DmRigor-mortis
    Depositor
    Elisa Izaurralde
    Insert
    DmRigor-mortis (rig Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    25 silent and 12 non-silent mutations compared to…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148203-plasmid-map-sequence-id-290651

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Showing: 4101 - 4120 of 12703 results