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pInducer10b-HA-KRAS G12V CO SR

Plasmid

#164928

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Mutation

Glycine 12 changed to valine; 9 Valine codons are…

Promoter

Ubc promoter

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-L536Q

Plasmid

#116369

Purpose

Lentiviral expression of ESR1 L536Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

L536Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-E380Q

Plasmid

#116368

Purpose

Lentiviral expression of ESR1 E380Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Mutation

E380Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPten#2/Cre

Plasmid

#173646

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-GNAS

Plasmid

#116748

Purpose

Lentiviral expression of GNAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GNAS (GNAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_WT

Plasmid

#82754

Purpose

Gateway Donor vector containing TP53, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TP53 (TP53 Human)

Use

Gateway entry vector

Mutation

P72R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_WT

Plasmid

#81734

Purpose

Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTp53#2/Cre

Plasmid

#173621

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR4

Plasmid

#116743

Purpose

Lentiviral expression of FGFR4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR4 (FGFR4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.T790M_p.L858R

Plasmid

#82914

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

T790M, L858R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTp53#3/Cre

Plasmid

#173622

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPten#1/Cre

Plasmid

#173645

Purpose

Expresses a Pten-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Pten (Pten Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK6

Plasmid

#116725

Purpose

Lentiviral expression of CDK6

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Q61R

Plasmid

#81652

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

Q61R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.G719D

Plasmid

#81708

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

G719D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS-Q61K

Plasmid

#116444

Purpose

Lentiviral expression of NRAS Q61K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Mutation

Q61K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCMVblast_SLC38A2_wt

Plasmid

#156180

Purpose

CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA se…

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SNAI1_WT

Plasmid

#82164

Purpose

Gateway Donor vector containing SNAI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SNAI1 (SNAI1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BTK_WT

Plasmid

#82197

Purpose

Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BTK (BTK Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS

Plasmid

#116767

Purpose

Lentiviral expression of NRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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