We narrowed to 11,118 results for: ARIA

1. pcpBpaRS_v2

   Plasmid

   #196485

   Purpose

   To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40(synthetase), Pol III tRNA promoter (tRNA), C…

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. IDE-2Strep

   Plasmid

   #210355

   Purpose

   Expresses IDE fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   IDE (IDE Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LacI-pLacI-pIV pm

   Plasmid

   #218101

   Purpose

   Phagemid that expresses LacI, and pIV under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pIV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATG9A-EGFP

   Plasmid

   #210367

   Purpose

   Expresses ATG9A fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-HA-KPTN

   Plasmid

   #87042

   Purpose

   mammalian expression of KPTN

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   KPTN (KPTN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-FLAG-Sestrin2

   Plasmid

   #72595

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

   Plasmid

   #170229

   Purpose

   Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAT9651-BEAR-GFP

   Plasmid

   #162989

   Purpose

   BEAR target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-HA-ITFG2

   Plasmid

   #87045

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)

    Plasmid

    #188379

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CABE-T3.1

    Plasmid

    #193267

    Purpose

    Expression of CABE-T3.1 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CABE-T3.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-SARS-CoV-2-Nsp3PLpro

    Plasmid

    #204208

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Use

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    PLpro of Nsp3

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3XFLAG_ALDOA in pCW57_tTA_Blast

    Plasmid

    #154210

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE repeats (dox off)

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VEGF binding scFv G6

    Plasmid

    #111716

    Purpose

    Yeast Surface Display of G6 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6
    

    Use

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDUET-GST AO7REwt

    Plasmid

    #213935

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Chicken ß-actin

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5-FLAG-KPTN

    Plasmid

    #87041

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CBE-T1.14

    Plasmid

    #193277

    Purpose

    Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.14
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-Sestrin2

    Plasmid

    #72593

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pcpAzpaRS_v2

    Plasmid

    #196483

    Purpose

    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV5.1-SARS-CoV-2 Nsp3

    Plasmid

    #204205

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Use

    Tags

    FLAGx3

    Expression

    Mammalian

    Mutation

    none, from obtained clone

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5 CASTOR1-Flag Cterm

    Plasmid

    #84488

    Purpose

    overexpression of CASTOR1 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

27. Flag-V0D1

    Plasmid

    #210348

    Purpose

    Expresses ATP6V0D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    V-ATPase V0D1 (ATP6V0D1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DEL-pHG165a

    Plasmid

    #90064

    Purpose

    LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    DEL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LacI-11/109-pHG165c

    Plasmid

    #90059

    Purpose

    Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LacI-11/109
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    109

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge