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We narrowed to 12,972 results for: BASE

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Showing: 8281 - 8320 of 12972 results

  2. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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  4. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  5. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  6. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

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  7. SUMO-PNGaseF

    Plasmid
    #228498
    Purpose
    10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    ngl
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228498-plasmid-map-sequence-id-455750

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  8. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54537-plasmid-map-sequence-id-127234

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  9. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

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  10. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

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  11. Clover2-C1

    Plasmid
    #54711
    Purpose
    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  13. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  14. dClover2-Cx43-7

    Plasmid
    #56532
    Purpose
    Localization: Gap Junctions, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
    Depositor
    Michael Davidson
    Insert
    Cx43
    Tags
    dClover2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pMH_AtCK2beta1_95-287_NoTag

    Plasmid
    #238520
    Purpose
    Bacterial expression of A. thaliana CK2beta1 95-287_NoTag
    Depositor
    Michael Hothorn
    Insert
    AtCK2beta1 (CKB1 Mustard Weed)
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238520-plasmid-map-sequence-id-477129

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  17. pMH_AtCK2alpha1_76-409_K146A_K148/149A_HC

    Plasmid
    #238516
    Purpose
    Bacterial expression of A. thaliana CK2alpha1 76-409_K146A_K148/149A_HC
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha1 (CKA1 Mustard Weed)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K146A_K148/149A
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238516-plasmid-map-sequence-id-477125

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  18. pNYCOMPS-C-Lic-His-FLAG

    Plasmid
    #182505
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    TEV-FLAG-10x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182505-plasmid-map-sequence-id-361391

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  19. pNYCOMPS-N-Lic-His-FLAG

    Plasmid
    #182506
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    10xHis-FLAG-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182506-plasmid-map-sequence-id-358073

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  20. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Available Since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

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  21. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  22. L4440

    Plasmid
    #1654
    Depositor
    Andrew Fire
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, T7p, lacZN, OriF1>>, OriF1<<
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1654-plasmid-map-sequence-id-422970

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  23. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Promoter
    pT7
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  24. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

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  25. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  26. pCODE-3

    Plasmid
    #247475
    Purpose
    Plasmid for inducible expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone
    Depositor
    Morten Norholm
    Insert
    argX, glyT, leuW, proL, argU, and ileX
    Expression
    Bacterial
    Mutation
    Rearrangement of tRNA operon, tRNA flanking regio…
    Promoter
    pTet
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247475-plasmid-map-sequence-id-491679

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  27. pMH_MBP_AtCCA1_88-198

    Plasmid
    #238517
    Purpose
    Bacterial expression of A. thaliana CCA1 88-198
    Depositor
    Michael Hothorn
    Insert
    AtCCA1 (CCA1 Mustard Weed)
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238517-plasmid-map-sequence-id-477133

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  28. pSG7084 pG46-46

    Plasmid
    #232621
    Purpose
    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).
    Depositor
    Stephan Gruber
    Type
    Empty backbone
    Use
    Precursor plasmid to generate modified dna circles
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    232621-plasmid-map-sequence-id-468938

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  29. dCas9-p300 HAT

    Plasmid
    #179544
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179544-plasmid-map-sequence-id-354774

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  30. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  31. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  34. pvPE-V4

    Plasmid
    #240288
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Article
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Insert
    pvPE-V4
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240288-plasmid-map-sequence-id-488805

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  35. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  40. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

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Showing: 8281 - 8320 of 12972 results