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We narrowed to 8,781 results for: construct

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Showing: 8281 - 8320 of 8781 results

  1. pMal_Abl-PRM 3R

    Plasmid
    #112086
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-3R (ABL1 Human)
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112086-plasmid-map-sequence-id-236089

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  4. pMAL-Abl-PRM 4R

    Plasmid
    #112087
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-4R (ABL1 Human)
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112087-plasmid-map-sequence-id-236084

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  6. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  13. pETDuet-TFIIE

    Plasmid
    #171083
    Purpose
    Co-expresses human TFIIE. The resulting plasmid was used to generate a single expression construct encoding 2 subunits, with His-tag on TF2E1.Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    TF2E1 (GTF2E1 Human)
    TF2E2 (GTF2E2 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171083-plasmid-map-sequence-id-335640

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  14. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  15. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid
    #208946
    Purpose
    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208946-plasmid-map-sequence-id-415680

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  16. pGL3Basic_ME.1/ApoEpromoter

    Plasmid
    #51435
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer
    Depositor
    Sohail Tavazoie
    Article
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Insert
    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    ME.1 enhancer is fused upstream of ApoE promoter …
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

    Plasmid
    #208958
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208958-plasmid-map-sequence-id-415784

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  21. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

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  22. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid
    #208947
    Purpose
    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208947-plasmid-map-sequence-id-415686

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  23. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid
    #122985
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains A58V,R73Q "milli" mutatio…
    Promoter
    human synapsin
    Available Since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122985-plasmid-map-sequence-id-238429

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  39. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

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  40. pAAV-UBC-S4-ExRaiCKAR

    Plasmid
    #160725
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S4-ExRaiCKAR
    Use
    AAV
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160725-plasmid-map-sequence-id-315528

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Showing: 8281 - 8320 of 8781 results