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R2Ct (1-520)

Plasmid

#115649

Purpose

expression of truncated Rag2 including the C-terminal region

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (1-520) (Rag2 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag2 (aa 1-520)

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1- Mst7-Erp2CT

Plasmid

#73922

Purpose

GST fusion- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1- Mst7-Erp1CT

Plasmid

#73920

Purpose

GST fusion- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 δCLD2α

Plasmid

#73874

Purpose

δCLD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Arcn1/ δCLD2α (ARCN1 Bovine)

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD2α

Plasmid

#73873

Purpose

Ret2LD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 CHI-7

Plasmid

#73869

Purpose

Chimaera 7 (COPD-Ret2)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Chimaera 7

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p415 CHI-1

Plasmid

#73863

Purpose

Chimaera 1 (Ret2-COPD)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Chimaera 1

Expression

Yeast

Promoter

Met25

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2

Plasmid

#73872

Purpose

Ret2 (full length)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2

Expression

Yeast

Promoter

Met25

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG325-Unc-9

Plasmid

#99084

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Unc-9 (also known as h.26.12f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Unc-9 (also known as h.26.12f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG246-MCP

Plasmid

#99083

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea MCP (also known as h.45.8g)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

MCP (also known as h.45.8g) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG245-Ras-related

Plasmid

#99079

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Ras-related (also known as h.18.4a) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG128-activin

Plasmid

#99075

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Activin (also known as activin 2)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Activin (also known as activin 2) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Expression

Worm

Promoter

none

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV-Zeo-NKX2-8

Plasmid

#75090

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp9-2

Plasmid

#73853

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
FCIV1-GR SS287AA

Plasmid

#72703

Purpose

Lentiviral expression of GR Phospho-deficient mutant (2nd generation)

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

GR (Nr3c1 Rat)

Use

Lentiviral

Tags

IRES-Venus

Expression

Mammalian

Mutation

SS287AA

Promoter

ubiquitin

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pHL 1410

Plasmid

#53011

Purpose

pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

DsrA sRNA
ompC
rpoS

Use

Synthetic Biology

Tags

T7noRBS (900bp) and gfp

Expression

Bacterial

Mutation

contains −150 to +30 bp relative to start codon a…

Promoter

pCon, pLlacO-1, and pLtetO-1

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pHL1801

Plasmid

#53039

Purpose

PLlacO-1:RBS(st7):gfp

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

GFP

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only