We narrowed to 9,083 results for: mel

1. MEK1 (S218D,S222D)-pcw107

   Plasmid

   #64604

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Mutation

   S218D, S222D

   Promoter

   PGK

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLentiCMVblast_SLC38A2_wt

   Plasmid

   #156180

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA se…

   Promoter

   CMV

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pINDUCER20_mStrawberry_Atg4BC74A

   Plasmid

   #161735

   Purpose

   Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   220-222 TGC (Cys) is changed to GCA (Ala)

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pIGF1_P1 Promoter-FLuc

   Plasmid

   #154266

   Purpose

   Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   IGF-1 canonical P1 Promoter (IGF1 Human)
   

   Expression

   Mammalian

   Promoter

   Human IGF-1 P1 Promoter

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR003-sgTP53-2

   Plasmid

   #118020

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-2 (TP53 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXPR003-sgTP53-4

   Plasmid

   #118022

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-4 (TP53 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXPR003-sgTP53-1

   Plasmid

   #118019

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-1 (TP53 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-cT3G-cERP2-HOPX

   Plasmid

   #192923

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOPX

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOPX (HOPX Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR003-sgTP53-3

   Plasmid

   #118021

   Purpose

   Constitutive expression of sgRNA targeting human TP53

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   sgTP53-3 (TP53 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid

    #161733

    Purpose

    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    

    Use

    Lentiviral; Destinatioin vector for gateway cloni…

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    220-222 TGC (Cys) is changed to GCA (Ala)

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB-cT3G-cERP2-KLF6

    Plasmid

    #192909

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF6 (KLF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MEK1-pcw107-V5

    Plasmid

    #64650

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    PGK

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pXPR003-sgTP53-5

    Plasmid

    #118023

    Purpose

    Constitutive expression of sgRNA targeting human TP53

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    sgTP53-5 (TP53 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIGF1_P2 Promoter-FLuc

    Plasmid

    #154267

    Purpose

    Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Expression

    Mammalian

    Promoter

    Human IGF-1 P2 Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB-cT3G-cERP2-KLF4

    Plasmid

    #192908

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of KLF4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    KLF4 (KLF4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCMVblast_SLC38A2_N82A

    Plasmid

    #156181

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    CMV

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIGF1_P1 Promoter-FLuc2P

    Plasmid

    #154261

    Purpose

    Destabilized firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 canonical P1 Promoter (IGF1 Human)
    

    Expression

    Mammalian

    Promoter

    Human IGF-1 P1 Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIGF1_P2 Promoter-FLuc2P

    Plasmid

    #154262

    Purpose

    Destabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    IGF-1 P2 Promoter (IGF1 Human)
    

    Expression

    Mammalian

    Promoter

    Human IGF-1 P2 Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge