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Plasmid#72929PurposeAllows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoterDepositorInsertalpha skeletal Tropomyosin
UseTagsExpressionBacterialMutationPromoterrhaBAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del1 pET28a
Plasmid#122115PurposeExpression Brg1 amino acids 1-381 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiB
Plasmid#68050PurposeStrain: AM5116, Used for protein purificationDepositorInsertKaiB
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3992 pET ubcH5
Plasmid#8652DepositorInsertUbcH5 (UBE2D1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delWGR-L713F
Plasmid#173941PurposeBacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutationDepositorInsertPARP1delWGR-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 521-660; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15-His6
Plasmid#201880PurposeUsed for bacterial expression of human protein ARL15DepositorInsertARL15 (ARL15 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only