Addgene

Showing: 4161 - 4180 of 28391 results

Results per page:

Sort results by:

pENTR-GFP-WT1 -KTS N terminal HA

Plasmid

#217641

Purpose

Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway cloning

Tags

HA

Expression

Mutation

p.408_410delKTS

Promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-eCas9

Plasmid

#140237

Purpose

Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

EF-1a core

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Use

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HA-ARHGAP29

Plasmid

#104154

Purpose

mammalian expression of HA tagged ARHGAP29

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 (ARHGAP29 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

Plasmid

#62911

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pQ79

Plasmid

#175064

Purpose

expresses mutant of the huntingtin exon1 in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

polyQ79 (HTT Human)

Use

Tags

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET17b-yebST

Plasmid

#175804

Purpose

Complementation of yebST knockout in E. coli K-12

Depositor

Damian Ekiert, Ian Henderson, Gira Bhabha

Article

Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

Insert

yebST

Use

Tags

None

Expression

Bacterial

Mutation

silent mutations to introduce restriction sites

Promoter

T7

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eGFP-KIF1A-Rigor

Plasmid

#172207

Purpose

Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 251 to Alanine

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Use

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

Plasmid

#168107

Purpose

PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

Cas9-P2A-mCherry

Use

CRISPR and Cre/Lox

Tags

HA

Expression

Mutation

Promoter

CAG

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFL-EGFP-3xFlag-DEST

Plasmid

#134903

Purpose

Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Type

Empty backbone

Use

Tags

3x-Flag

Expression

Insect

Mutation

Promoter

polyhedron

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge