We narrowed to 26,423 results for: CAL

1. MAC_C_PDK1

   Plasmid

   #111672

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PDK1 (PDK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-Cre

   Plasmid

   #125574

   Purpose

   Expresses Cre under the CAG promoter in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-DDK

   Plasmid

   #137764

   Purpose

   Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GFP
   

   Use

   Tags

   DDK

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cr3Vn

   Plasmid

   #184861

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. gRNAy

   Plasmid

   #168294

   Purpose

   Expresses gRNA targeting the yellow gene in Drosophila

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   U6.3-gRNA[y]-scaffold
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-FLAG-LOV*-MVP

   Plasmid

   #202206

   Purpose

   Expresses the photoproximity labeling catalyst LOV* fused to MVP in mammalian cells

   Depositor

   Thomas Muir

   Article

   Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

   Insert

   Major vault protein (MVP Human)
   

   Use

   Tags

   FLAG and LOV*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-NLS-PhoCl-mCherry

   Plasmid

   #87691

   Purpose

   Express NLS-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NLS-PhoCl-mCherry
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPRv2-sgMaob-HepmCherry

   Plasmid

   #192829

   Purpose

   Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Myc-GRM1-IRES-CyRFP1

   Plasmid

   #195355

   Purpose

   mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

   Depositor

   Brenda Bloodgood

   Article

   Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

   Insert

   GRM1-IRES-CyRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pH6HTC_ENO1-Halo

    Plasmid

    #175330

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ENO1 (ENO1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ER50-SpCas9-ER50

    Plasmid

    #85448

    Purpose

    Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    ER50-SpCas9-ER50
    

    Use

    AAV

    Tags

    destabilized domain of estrogen receptor ligand b…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentiCRISPRv2-Stuffer-HepmCherry

    Plasmid

    #192825

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #126218

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCTCON2-BYG

    Plasmid

    #158126

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    mTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LDB-3-p65

    Plasmid

    #158984

    Purpose

    Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-3-p65
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1a-PTDSS1-IRES-Puromycin

    Plasmid

    #134667

    Purpose

    Expresses EGFP-tagged Mitochondria-associated ER membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    PTDSS1 (PTDSS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CAG_mCh_p85a_mCh

    Plasmid

    #199630

    Purpose

    FRET acceptor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    p85a-SH2 (Pik3r1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

    Plasmid

    #199346

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTEN shRNA

    Plasmid

    #86645

    Purpose

    expression of shRNA targeting PTEN

    Depositor

    Jaime Modiano

    Article

    Lin et al Mol Cancer. 2007 Feb 14;6:16.

    Insert

    PTEN (PTEN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-UBE2E1 C131A in pcDNA3.1

    Plasmid

    #118214

    Purpose

    Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed cysteine 131 to alanine

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DHFR-PP7-VP64_T2A_GFP

    Plasmid

    #86167

    Purpose

    Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-PP7-VP64_T2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized domain (N-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_VDR_Hormone-recep

    Plasmid

    #109962

    Purpose

    Protein expression and purification of VDR_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VDR_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. [pSK104] pET28 SLC38A9N-His

    Plasmid

    #136142

    Purpose

    bacterial expression of SLC38A9 N term fragment (1-119)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Plasmid

    #163075

    Purpose

    Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SHC003BSD-DelGFP

    Plasmid

    #133301

    Purpose

    EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHH0103_PFN1_Profilin

    Plasmid

    #110028

    Purpose

    Protein expression and purification of PFN1_Profilin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PFN1_Profilin (PFN1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

    Plasmid

    #55222

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB-CMV-MCS-Puro GEM-LTB4mut

    Plasmid

    #202642

    Purpose

    Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    GEM-LTB4mut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. VinculinTS-A50I/I997A

    Plasmid

    #111829

    Purpose

    Vinculin tension sensor with point mutations affecting ability to bind talin and actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed vinculin alanine 50 to isoleucine and iso…

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV-PKCb1-mEGFP-N1

    Plasmid

    #112265

    Purpose

    ITRACKbeta/IDOCKSbeta Donor construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c beta (Prkcb Mouse)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJAK112

    Plasmid

    #167280

    Purpose

    CodA-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CAG:: mCherry-2a-hM4Dnrxn

    Plasmid

    #52523

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    mCherry-2a-hM4Dnrxn
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAg

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

    Plasmid

    #139961

    Purpose

    Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAG1280 NLS-mCherry-FIREtag-NES

    Plasmid

    #205172

    Purpose

    expresses NLS-mCherry-FIREtag-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMF-FU_TetO_GW_PPARG2

    Plasmid

    #193289

    Purpose

    Lentiviral construct that expresses a dox-inducible human PPARG2

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    PPARG2 (PPARG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_RANBP1_Ran-BP1

    Plasmid

    #109872

    Purpose

    Protein expression and purification of RANBP1_Ran-BP1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RANBP1_Ran-BP1 (RANBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pH6HTC_IGF2BP2a-Halo

    Plasmid

    #175309

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP2 (IGF2BP2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2-mCherry_SNAP_SNAPV_PACT_Active

    Plasmid

    #173072

    Purpose

    Active SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge