We narrowed to 18,278 results for: Met;

1. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CaMKIIa-eArchT 3.0-EYFP

   Plasmid

   #35513

   Purpose

   Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   eArchT 3.0-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Enhanced trafficking signal and ER export signal …

   Promoter

   CaMKIIa

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTag-BFP-C-h-Rab4a-c-Myc

   Plasmid

   #79799

   Purpose

   Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab4a (RAB4A Human)
   

   Use

   Tags

   TagBFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PA-mCherry1-Mito-7

   Plasmid

   #57189

   Purpose

   Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 10XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111547

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mIFP-H2B-C-10

   Plasmid

   #56224

   Purpose

   Localization: Nucleus/Histones, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pAAV-EF1a-sCre

   Plasmid

   #55635

   Purpose

   Expresses sCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

   Plasmid

   #197885

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. DRH335: FCK-Optopatch1

   Plasmid

   #51695

   Purpose

   Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   Optopatch1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-VTKD2

    Plasmid

    #170847

    Purpose

    AAV backbone for Cre-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH_TNF-SBP-EGFP

    Plasmid

    #65282

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. scFv-GCN4-DNMT3a-DNMT3l

    Plasmid

    #154140

    Purpose

    Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

    Plasmid

    #35507

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS18 (mANGPTL3-Strep)

    Plasmid

    #109111

    Purpose

    Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (Angptl3 Mouse)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dronpa-DEVD-TagRFPT

    Plasmid

    #87704

    Purpose

    Fluorescent biosensor for super-resolution imaging of caspase activity.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Dronpa-DEVD-TagRFPT
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

    Plasmid

    #197893

    Purpose

    Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

    Plasmid

    #98930

    Purpose

    AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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