We narrowed to 6,654 results for: SIM;

1. pROF394

   Plasmid

   #155333

   Purpose

   Expression of PULSE-controlled LFY-VP16-2A-RLuc

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   (etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
   

   Use

   Synthetic Biology; Eukaryotic expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159872

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETM33_PEX14_Pex14

   Plasmid

   #178484

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   PEX14_Pex14 (PEX14 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK0_047

   Plasmid

   #123977

   Purpose

   Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hCLYBL CAS9 Destination - HygroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV Gab1(PRD)-mCherry-HOTag3

   Plasmid

   #190026

   Purpose

   Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   Gab1(PRD) CluMPS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK0_057

   Plasmid

   #123950

   Purpose

   Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI LP on hAAVS1 (Hygro::mRuby)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TB:6xAP-1_RE:MiniP

   Plasmid

   #133884

   Purpose

   6 copies of the AP-1 binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   6 copies of the AP-1 binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gi-c009

   Plasmid

   #173079

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   GNAI1 (GNAI1 Human)
   

   Use

   Tags

   mCitrine

   Expression

   Bacterial

   Mutation

   full length, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-BirA-CPDSalI

   Plasmid

   #38258

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   birA (birA E. coli)
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pGEX4T1-BirA

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pQStrep2-CRYM

    Plasmid

    #31293

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    crystallin, mu (CRYM Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Use

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSI-535

    Plasmid

    #131130

    Purpose

    gRNA expression vector for C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    C-to-T reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-UFD1L

    Plasmid

    #31560

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    ubiquitin fusion degradation 1-like (UFD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P[acman]-Omega2

    Plasmid

    #165861

    Purpose

    Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159847

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MDO2-c003

    Plasmid

    #173105

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K7-D243

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MDO2-c005

    Plasmid

    #173106

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    K7-D243

    Promoter

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCph8

    Plasmid

    #22869

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

    Insert

    pCph8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 517 amino acids of Cph1 and the last 229 am…

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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