We narrowed to 12,703 results for: sequence

1. pAc5.1B-lambdaN-HA-DmSmaug-V5His6_C

   Plasmid

   #146017

   Purpose

   Insect Expression of DmSmaug

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug (smg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   10 silent mutations and a deletion of the AA 961-…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmUPF3-K31E-siRNAres-V5His6_N

   Plasmid

   #147007

   Purpose

   Insect Expression of DmUPF3-K31E-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF3-K31E-siRNAres (Upf3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   5 silent and one non silent mutation P305S compar…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM-30-GST-DmGW182_1-539_F

   Plasmid

   #146264

   Purpose

   Bacterial Expression of DmGW182_1-539

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-539 (gw Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   one non silent mutation G349A (G117R) compared to…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

   Plasmid

   #146166

   Purpose

   Insect Expression of DmUPF2-siRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmUPF2-siRNAres (Upf2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   3 non silent mutations: L1022P, V1097L and N1213S…

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

   Plasmid

   #146001

   Purpose

   Insect Expression of DmSmaug_1-762

   Depositor

   Elisa Izaurralde

   Insert

   DmSmaug_1-762 (smg Fly)
   

   Use

   Tags

   V5-6xHis

   Expression

   Insect

   Mutation

   NP_523987.1, 7 silent mutations compared to the s…

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. T-FAM83BA-6

   Plasmid

   #166551

   Purpose

   scFv of a human scaffold targeting Protein FAM83B. Antigen coverage aa 117-294 of 1011

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. O-AASSA-25

   Plasmid

   #166537

   Purpose

   scFv of a human scaffold targeting Aminoadipate-semialdehyde synthase. Antigen coverage aa 455-926 of 926

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. J-CDAN1A-5

   Plasmid

   #166522

   Purpose

   scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Z-OPTN-10

   Plasmid

   #166562

   Purpose

   scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV6-Bmal-luc

    Plasmid

    #68833

    Purpose

    Expression of Bmal1-luc in mammalian cells

    Depositor

    Steven Brown

    Insert

    Bmal1 regulatory sequences (Bmal1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220735

    Purpose

    AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Insert

    iCre(R297T)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid

    #212705

    Purpose

    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

    Depositor

    Elzo de Wit

    Insert

    ZFP143-FKBPV-2HA-P2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-mCardinal-SKL

    Plasmid

    #183728

    Purpose

    For exrpression of mCardinal targeted to peroxisomes

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Insert

    mCardinal
    

    Use

    Lentiviral

    Tags

    SKL (peroxisomal targeting sequence 1)

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_SFFV*/Puro2a-GFP

    Plasmid

    #198222

    Purpose

    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP

    Depositor

    Eric Davis

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Codon-optimized puromycin resistance gene, follow…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. O-TREM2-2

    Plasmid

    #166545

    Purpose

    scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOpen-ETSSB

    Plasmid

    #165549

    Purpose

    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Extreme Thermostable Single-Stranded DNA Binding Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pPGKFLPobpA

    Plasmid

    #13793

    Purpose

    Expression of mouse codon-optimized FLP recombinase with bovine growth hormone polyadenylation (bpA) sequence.

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS ONE. 2007 . 2():e162.

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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