We narrowed to 250 results for: lentivirus expression plasmid

1. pLEX307-MERS-CoV-3CL-C148A

   Plasmid

   #160283

   Purpose

   Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C148A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX307-IBV-3CL-C143A

   Plasmid

   #160289

   Purpose

   Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C143A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187611

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

   Plasmid

   #187612

   Purpose

   Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Inserts

   WWE domain (R163A) with C-terminal nLuc
   WWE domain with C-terminal cLuc
   

   Use

   Lentiviral

   Tags

   FLAG, HA, Split luciferase (cLuc), Split lucifera…

   Expression

   Mammalian

   Mutation

   Arg163Ala

   Promoter

   EF1A

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107311

   Purpose

   Encoding scFv-p65-HSF1 driven by EF1α promoter

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBOBI-FLuc

   Plasmid

   #170674

   Purpose

   Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   luc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

   Plasmid

   #216164

   Purpose

   Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   Gaussia princeps luciferase (with double mutation to improve stability)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-REGv2 Cryptic PE-Max

   Plasmid

   #216163

   Purpose

   Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   PEMax for prime editing with internal cryptic exon
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pinducer 20 DN-KASHΔPPPL

   Plasmid

   #129280

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

   Depositor

   Daniel Conway

   Article

   Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Expression

   Mutation

   the last 4 amino acids (PPPL) of the KASH domain …

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p53 (dominant negative R175H mutant)-pcw107-V5

    Plasmid

    #64638

    Purpose

    3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    TP53 (transcript variant 3) (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    R175H

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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