Skip to main content
Addgene
Showing: 1001 - 1050 of 158806 results
  1. pGMGUS

    Plasmid
    #115678
    Purpose
    E.coli-Streptomyces shuttle vector, for gene knock out experiments
    Depositor
    Insert
    gusA, aac(3)IV, tsr
    Use
    Gene knock out
    Tags
    Expression
    Mutation
    Promoter
    ermE*
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pHyVec11

    Plasmid
    #34794
    Depositor
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    Intergenic region between Hydra replication facto…
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
  3. pwAC-trAO217erg12

    Plasmid
    #227347
    Purpose
    Overexpression of the truncated AO217 and erg12 genes in Aspergillus oryzae
    Depositor
    Insert
    Expression cassettes of the truncated AO217 and erg12 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pSARGENT1

    Plasmid
    #216315
    Purpose
    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence
    Depositor
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pHyVec7

    Plasmid
    #34793
    Depositor
    Type
    Empty backbone
    Use
    Hydra expression
    Tags
    GFP, RFP, and intergenic region between Hydra rep…
    Expression
    Mutation
    Promoter
    Hydra vulgaris actin promoter
    Available since
    Jan. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
  6. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pENTR-Hygro

    Plasmid
    #139555
    Purpose
    Hygromycin resistant gene ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Insert
    Hygromycin resistant gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. 1162BX-CMV-EGFP-bGHpolyA

    Plasmid
    #235171
    Purpose
    Expresses EGFP
    Depositor
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pwAB-erg8erg19idi1erg20

    Plasmid
    #227345
    Purpose
    Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae
    Depositor
    Insert
    Expression cassettes of the erg8, erg19, idi1, and erg20 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pdadhDN-tefAO217

    Plasmid
    #227341
    Purpose
    Replacement of the adh gene with the expression cassette of the AO217 gene
    Depositor
    Insert
    Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. ppG-ple12

    Plasmid
    #227339
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Insert
    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pwA-ple34

    Plasmid
    #227337
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Insert
    The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pDONR221-RBM11

    Plasmid
    #192748
    Purpose
    Gateway entry vector encoding human RBM11
    Depositor
    Insert
    RBM11 (RBM11 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. Hg

    Plasmid
    #107573
    Purpose
    4 kb HindII rat genomic fragment containing the entire VGF gene
    Depositor
    Insert
    genomic fragment containing the entire VGF gene (Vgf Rat)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. 8xNFAT-ZsG-muhCD4

    Plasmid
    #162745
    Purpose
    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene
    Depositor
    Insert
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Human CD4 gene with glutamine to tyrosine at posi…
    Promoter
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pLCKO2

    Plasmid
    #125518
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pEMS2115

    Plasmid
    #49140
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Insert
    ssAAV-Ple155-emGFP-WPRE
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Use
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  19. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179436
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pC-BAR-YR

    Plasmid
    #61766
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).
    Depositor
    Inserts
    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  22. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid
    #193253
    Purpose
    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Depositor
    Insert
    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    Use
    Lentiviral
    Tags
    Renilla luciferase (rLuc) and yellow fluorescent …
    Expression
    Mutation
    None
    Promoter
    EF1a
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  23. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  24. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  25. pEMS1985

    Plasmid
    #49116
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Insert
    ssAAV-Ple155-iCre
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  26. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid
    #123180
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
    Insert
    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    Use
    Luciferase; Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1050 of 158806 results