We narrowed to 11,286 results for: 110

1. pBTK614

   Plasmid

   #110608

   Purpose

   BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_RAC1_Ras

   Plasmid

   #110030

   Purpose

   Protein expression and purification of RAC1_Ras

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RAC1_Ras (RAC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2 you

   Plasmid

   #11084

   Depositor

   William Talbot

   Article

   Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

   Insert

   you (scube2 Zebrafish)
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pCZ829

   Plasmid

   #110137

   Purpose

   Epidermal Mitochondrial-tagged Florescent Calcium Sensor

   Depositor

   Andrew Chisholm

   Article

   Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

   Insert

   mito-GCaMP5
   

   Use

   Tags

   mito::GCaMP5

   Expression

   Worm

   Mutation

   Promoter

   Pcol-19

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCOND (Hyg)

   Plasmid

   #110093

   Purpose

   Suicide vector based on the TetR/Pip OFF system.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1alpha-HA-TFAP4

   Plasmid

   #110158

   Purpose

   Lentiviral vector for expression of human TFAP4 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK401

   Plasmid

   #110597

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCC336

   Plasmid

   #110167

   Purpose

   miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   Phospho-acceptor sites mutated: S43E, T55E, T62E.…

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCC335

   Plasmid

   #110166

   Purpose

   miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   Phospho-acceptor sites mutated: S43A, T55A, T62A.…

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tet-pLKO.puro_shHuR 3UTR

    Plasmid

    #110412

    Purpose

    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAVL1 (HuR)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO (RNA PolIII)

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. cfSBFP2-N

    Plasmid

    #110633

    Purpose

    Cys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.

    Depositor

    Ikuo Wada

    Insert

    cfSBFP2
    

    Use

    Tags

    Expression

    Mutation

    Cys48Ser/Cys70Met

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_PLAT_Trypsin

    Plasmid

    #110039

    Purpose

    Protein expression and purification of PLAT_Trypsin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PLAT_Trypsin (PLAT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHH0103_PFN2_Profilin

    Plasmid

    #110007

    Purpose

    Protein expression and purification of PFN2_Profilin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PFN2_Profilin (PFN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_ACR_Trypsin

    Plasmid

    #110042

    Purpose

    Protein expression and purification of ACR_Trypsin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ACR_Trypsin (ACR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mature ISG15 C-term. domain (79-157)

    Plasmid

    #110764

    Purpose

    Bacterial expression of proISG15 C-term. domain

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK300

    Plasmid

    #110592

    Purpose

    BTK Type 4 terminator for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    rpoC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCZGY2162

    Plasmid

    #110136

    Purpose

    Epidermal Mitochondrial-tagged Florescent Calcium Sensor

    Depositor

    Andrew Chisholm

    Article

    Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

    Insert

    mito::GCaMP3
    

    Use

    Tags

    mito::GCaMP3

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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