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pcDNA-Prom1 D-5

Plasmid

#184017

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBScMXT + mSlo1 MBr5/3

Plasmid

#16149

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse brain)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pBScMXT + mSlo1 MBr5/23

Plasmid

#16151

Depositor

Larry Salkoff

Article

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214889

Purpose

Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214890

Purpose

Expresses the optogenetic tool BiPOLES in BWMs of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB17 (punc-17::QuasAr)

Plasmid

#214888

Purpose

Expresses the voltage sensor QuasAr2 in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

QuasAr2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

Plasmid

#214891

Purpose

Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB9v2

Plasmid

#210029

Purpose

Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB1163

Plasmid

#210032

Purpose

Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas11-P2A-Cas6-T2A-Cas3
rtta-T2A-blast

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB758

Plasmid

#210033

Purpose

Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas7-P2A-Cas5-T2A-Cas8
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE033

Plasmid

#176790

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

402

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE043

Plasmid

#176800

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

412

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D-4

Plasmid

#184016

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 651 to 665.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-4 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D-6

Plasmid

#184018

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 631 to 645.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-6 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D+3

Plasmid

#184022

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 721 to 735.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D+3 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D+1

Plasmid

#184020

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 702-716.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D+1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D-7

Plasmid

#184019

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 598-692.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-7 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 SV8 (-Ex19a)

Plasmid

#184012

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701).

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Prom1 D+2

Plasmid

#184021

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 711 to 725.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D+2 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge