Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 456 results for: Argonaute

Showing: 401 - 420 of 456 results

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pLJM_3xHA_HsAGO2(R315V, H316A)

Plasmid

#247115

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

R315V, H316A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pLJM_3xHA_HsAGO2(d296-305)

Plasmid

#247116

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

d296-305

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pLJM_3xHA_HsAGO2(d332-336)

Plasmid

#247117

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

d332-336

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-Zeo-miR-430

Plasmid

#221726

Purpose

Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO1

Plasmid

#247137

Purpose

Expression of AGO for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute1 (AGO1 Human, Synthetic)

Tags

3xFLAG-SUMO(Eu1)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pLJM_3xHA_HsAGO2(E464A, V465A, K468A)

Plasmid

#247123

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

E464A, V465A, K468A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pLJM_3xHA_HsAGO2(K335A, H336A, T337A)

Plasmid

#247118

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

K335A, H336A, T337A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(F294A)

Plasmid

#247131

Purpose

Expression of AGO for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO(Eu1)

Expression

Mammalian

Mutation

F294A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM_3xHA_HsAGO2(H77A, H81A, T118A, P120A)

Plasmid

#247128

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

H77A, H81A, T118A, P120A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM_3xHA_HsAGO2(F491A, K493A, M504A, H507A)

Plasmid

#247122

Purpose

Expression of AGO to assay in-cell microRNA stability

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

3xHA

Mutation

F491A, K493A, M504A, H507A

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 420 of 456 results

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