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pMXs-EGFP-Rheb-HVR HRas-IP

Plasmid

#13833

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb-HVR HRas (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminal of Rheb and hyper variable region (HVR…

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#67608

Purpose

bacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

catalytic mutant LDIG(182-185)AAAA

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-Rab4a-7

Plasmid

#55507

Purpose

Localization: Ras GTPase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Rab4a (RAB4A Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-H4-23

Plasmid

#55491

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H4 (HIST1H4J Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Alpha-Actinin-19

Plasmid

#55464

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

PAH1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-CDS1-3HA

Plasmid

#184760

Purpose

CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CDS1 (CDS1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

CDS1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MTK3_028

Plasmid

#124054

Purpose

Encodes c120 sg RNA guide cleaved by ribozyme

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RIbozyme (C120 sg RNA)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ePsuACR-mRuby2-ST

Plasmid

#109133

Purpose

Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ePsuACR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eiC++-mRuby2-ST

Plasmid

#109132

Purpose

Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eiC++-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWHI2-HYG

Plasmid

#80589

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Expression

Yeast

Promoter

WHI2 native promoter

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 deltaC 350 (stop)

Plasmid

#67612

Purpose

bacterial expression of GST-PRMT9 missing residues 351-845

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codons placed at aa 351 and 352 to create C …

Promoter

tac

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Talin-ABM-10

Plasmid

#55510

Purpose

Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Talin ABM

Tags

mTFP1

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCeSR beta deltaN

Plasmid

#13728

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (R186.3 Nematode)

Tags

6xHis

Expression

Bacterial

Mutation

expresses residues 38-240 of C. elegans SRβ with …

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SauriCas9

Plasmid

#135964

Purpose

Expresses SauriCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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