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HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 2

Plasmid

#8471

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

261DLF263-AAA (D261A, L262A, F263A)

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Use

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-p107 2*

Plasmid

#32388

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

2nd E2F binding site mutated: GTC-->AAA

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJB51

Plasmid

#226308

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1

Use

Tags

Expression

Bacterial

Mutation

VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Use

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Use

Tags

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCtnnd2.2-GFP

Plasmid

#209098

Purpose

GFP expressing scramble of shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MYMH 351

Plasmid

#138058

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-10AG 11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG GCA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 327

Plasmid

#138054

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC-GFP

Use

Tags

Expression

Bacterial

Mutation

GCA GAG GAG AAA

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 67

Plasmid

#138048

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-11AT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG ATA

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 17

Plasmid

#138023

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-3AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAC GAG GAG AAA

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 73

Plasmid

#138051

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-12AT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG AAT

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 71

Plasmid

#138050

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-12AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG AAC

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 69

Plasmid

#138049

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-12AG-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG AAG

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 65

Plasmid

#138047

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-11AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG ACA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 63

Plasmid

#138046

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-11AG-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG AGA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 61

Plasmid

#138045

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-10AT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GAG TAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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