We narrowed to 159,238 results for: addgene

1. 6NEY

   Plasmid

   #127295

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD3
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 26, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. 6MF9

   Plasmid

   #127290

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD12
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pUPD1:P1-MAR

   Plasmid

   #106213

   Purpose

   Provides Glycine max P1 matrix attachment region as a level 0 GoldenBraid part

   Depositor

   Tomáš Moravec

   Insert

   Glycine max P1 matrix attachment region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 5CBD

   Plasmid

   #69970

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PVX_084705:MAC04G-C11:C228845
   

   Use

   Baculovirus expression

   Tags

   His and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 5C4Q

   Plasmid

   #69963

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   LdBPK_363130.1:MAC055-F03:C235029
   

   Use

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. XE174 Xenopus Wnt-5A-myc-pCDbeta-G

   Plasmid

   #16873

   Depositor

   Randall Moon

   Insert

   Wnt-5A (wnt5a.S Frog)
   

   Use

   Xenopus expression

   Tags

   myc

   Expression

   Mutation

   Promoter

   Available since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. XE176 Xenopus Wnt-8-myc-pCDbeta-G

   Plasmid

   #16864

   Depositor

   Randall Moon

   Insert

   Wnt-8 (wnt8a.L Frog)
   

   Use

   Xenopus expression

   Tags

   myc

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. ShuttleNSE

   Plasmid

   #50958

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NSE

   Available since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-IARS2-H112A/P115A

    Plasmid

    #232338

    Purpose

    Expresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Putative isoleucine binding deficiency mutation H…

    Promoter

    CMV

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-IARS2-K664A/K667A

    Plasmid

    #232339

    Purpose

    Expresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Putative tRNA binding deficiency mutation K664A A…

    Promoter

    CMV

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLBS-mScarlet

    Plasmid

    #129337

    Purpose

    cytosol marker, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plenti guide Thy1.1

    Plasmid

    #128063

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid

    #175571

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX551-CMV-SpCas9

    Plasmid

    #107024

    Purpose

    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab

    Depositor

    Alex Hewitt

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eIF2a 1

    Plasmid

    #21807

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eIF2a 2

    Plasmid

    #21808

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S52A

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge