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pET21a.MJ0882

Plasmid

#11417

Depositor

Sung-Hou Kim

Publication

Huang et al J Struct Funct Genomics. 2002 . 2(3):12

Insert

methyltransferase (MJ_0882 M. jannaschii)

Expression

Bacterial

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pSJ78 Phagemid with tetA

Plasmid

#254000

Purpose

Phagemid for LySE, encoding a tetracycline efflux protein for evolution.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

tetA

Expression

Bacterial

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-PKG-hNo.7

Plasmid

#175812

Purpose

The lentiviral vector for PKG #7, a genetically encoded FRET biosensor for monitoring cGMP concentration.

Depositor

Michiyuki Matsuda

Publication

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

PKG #7

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0697

Plasmid

#11411

Depositor

Sung-Hou Kim

Publication

Wang et al EMBO J. 2000 Feb 1. 19(3):317-23.

Insert

fibrillarin homolog (MJ_0697 M. jannaschii)

Expression

Bacterial

Mutation

M222T

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c26

Plasmid

#242875

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, A369P, 434G)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, A369P, 434G

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pZG71

Plasmid

#202577

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD mPlum

Plasmid

#91773

Purpose

Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Insert

monomeric Plum

Tags

no tag

Expression

Bacterial

Promoter

araBAD

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-cascade

Plasmid

#247069

Purpose

L-arabinose inducible E.coli cascade operon inserted in pCDF vector

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

E.coli Type I-E CRISPR cas

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

Plasmid

#244218

Purpose

Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

Plasmid

#244217

Purpose

Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-P300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

Plasmid

#244219

Purpose

Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PCR4-beta-globin::lacZ-H11

Plasmid

#227000

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

Depositor

Guillaume Andrey

Publication

Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

beta-globin

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSX018 BAC-phagemid CmR(TAG)

Plasmid

#253999

Purpose

BAC-phagemid for LySE, carrying CmR(TAG) for fluctuation analysis. Yeast origin (CEN) and selection (URA3) facilitates assembly in yeast.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

CmR(TAG)

Expression

Yeast

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pPBG01 Recombination plasmid

Plasmid

#253997

Purpose

Inducible lambdaRED for genomic recombination.

Depositor

Julius Fredens

Publication

Ong et al Nat Microbiol. 2026 May 1. doi: 10.1038/

Insert

lambdaRED

Use

Synthetic Biology

Expression

Bacterial

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1_star

Plasmid

#241277

Purpose

Orthogonal PYR1/HAB1 sensor module

Depositor

Sean Cutler

Publication

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

HAB1_star (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV Bxb1 attP dsRed Hygro

Plasmid

#242884

Purpose

Selectable donor plasmid for insertion by Bxb1 integrase into pre-inserted attB landing pad site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Hygromycin-IRE2S-DsRED Express 2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shAtg7-EGFP

Plasmid

#227684

Purpose

Expresses EGFP and shRNA targeting Atg7

Depositor

Cagla Eroglu

Publication

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a Bxb1 attB38

Plasmid

#242878

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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