We narrowed to 524 results for: hyper

1. pCMV-Tag2B EDD C2768A

   Plasmid

   #37189

   Depositor

   Charles Watts, Darren Saunders

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD C2768A (UBR5 Human)
   

   Use

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   Changed Cysteine 2768 to Alanine

   Promoter

   CMV

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC/CMV-TYK2V678F-VSV

   Plasmid

   #139349

   Purpose

   Expression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)

   Depositor

   Sandra Pellegrini

   Article

   Gakovic et al J Biol Chem. 2008 Jul 4;283(27):18522-9.

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F-V678F

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast POLD1

   Plasmid

   #160805

   Purpose

   Express POLD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLD1 (POLD1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized C-terminal 230 amino acids

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-RFP-UCR1C

   Plasmid

   #67582

   Purpose

   Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

   Insert

   C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
   

   Use

   Tags

   FLAG and TagRFP

   Expression

   Mammalian

   Mutation

   Amino acid residues 80-136 of PDE4D3

   Promoter

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. B52_puro_gRNA_3418711_KCNB2

   Plasmid

   #197558

   Purpose

   gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

   Depositor

   Darius Bägli

   Insert

   upstream KCNB2 promoter gRNA (Kcnb2 Rat)
   

   Use

   Grna with puromycin selection

   Tags

   puromycin resistance cassette under CMV promotion

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSPL3-ABCC8-2

   Plasmid

   #135916

   Purpose

   Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPL3-ABCC8-9-10

   Plasmid

   #135918

   Purpose

   Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast POLE1 C-ISCmut

    Plasmid

    #160804

    Purpose

    Express C-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 C-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSPL3-ABCC8-27

    Plasmid

    #135919

    Purpose

    Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Flag-PGC-1alpha2

    Plasmid

    #45502

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform2 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-Blast POLD1 ISCmut

    Plasmid

    #160806

    Purpose

    Express ISC mutant POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 ISCmut (POLD1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon optimized C-terminal 230 amino acids, C1058…

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-Blast POLA1 ISCmut

    Plasmid

    #160808

    Purpose

    Express ISC mutant POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLA1 ISCmut (POLA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Codon optimized, C1354S, C1359S, C1377S, C1380S

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

    Plasmid

    #159679

    Purpose

    Mammalian protein expression of KRAS4b-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.KRAS4b (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R713-M88-303: CMV51p> Halotag-Hs.HRAS

    Plasmid

    #159681

    Purpose

    Mammalian protein expression of HRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.HRAS (HRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R713-M91-303: CMV51p> Halotag-Hs.NRAS

    Plasmid

    #159682

    Purpose

    Mammalian protein expression of NRAS-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Hs.NRAS (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTripz NEO DEST

    Plasmid

    #209072

    Purpose

    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

    Depositor

    Matthew Oser

    Article

    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector to be used…

    Tags

    3X FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge