We narrowed to 1,631 results for: prep

1. pLX-SFFV_NKX2-1

   Plasmid

   #221496

   Purpose

   NKX2-1 short isoform lentiviral overexpression using SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1419

   Plasmid

   #219778

   Purpose

   pDEF-TurboID-GFPmut2, negative control for cytosolic expression

   Depositor

   Scott Lindner

   Article

   Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

   Insert

   TurboID/GFPmut2
   

   Use

   Plasmodium, rodent malaria parasites

   Tags

   Expression

   Mutation

   Promoter

   PbEF1alpha promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Efg1-ΔDBD

   Plasmid

   #216396

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 182-356

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Efg1-N-PrLD

   Plasmid

   #216397

   Purpose

   Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-N-PrLD
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 182-554

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-ODN-hsRPL3-CterTwStrep

   Plasmid

   #208644

   Purpose

   for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
   

   Use

   CRISPR; Ssdna donnor preparation

   Tags

   Twin Strep

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGM9121_Strep II

   Plasmid

   #153512

   Purpose

   Strep II C-terminal Tag Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   Strep II
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM9121_TwinStrep

   Plasmid

   #153513

   Purpose

   TwinStrep C-terminal Tag Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   TwinStrep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM9121_Aaw-Glucoamylase SP

   Plasmid

   #153497

   Purpose

   Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   Glucoamylase SP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGM9121_Ani- α Amylase SP

   Plasmid

   #153503

   Purpose

   Aspergillus niger α Amylase Signal Peptide Donor Plasmid

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   α Amylase SP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM9121_Sce- α Galactosidase SP

    Plasmid

    #153504

    Purpose

    α Galactosidase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    α Galactosidase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM9121_Gga-Lysozym SP

    Plasmid

    #153505

    Purpose

    Chicken Lysozym Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Lysozym SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAP003

    Plasmid

    #153490

    Purpose

    Level 2 integrative plasmid for stable expression in P. pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGM9121_Sce-Invertase 2 SP

    Plasmid

    #153493

    Purpose

    S. cerevisiae Invertase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Invertase 2 SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJBL7007

    Plasmid

    #136946

    Purpose

    Malachite green aptamer with stability hairpin, bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    malachite green aptamer (MGA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMON-HsNMT1

    Plasmid

    #67475

    Purpose

    Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    HsNMT1 (NMT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GT_CAG-mRuby2

    Plasmid

    #229801

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    V74A, E229K, V375I

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIVT-Flp-2A-BleoR

    Plasmid

    #232752

    Purpose

    IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Inserts

    FlpO
    BleoR
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge