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pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

Plasmid

#118274

Purpose

Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

bPAC(WT)
SthK

Use

AAV

Tags

His tag, P2A, mCherry, and myc tag

Expression

Mammalian

Promoter

CamK II

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

Plasmid

#218753

Purpose

Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

Plasmid

#211818

Purpose

AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

C-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

Plasmid

#89570

Purpose

Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

Depositor

McLean Bolton

Article

Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

Inserts

Channelrhodopsin
Histone 2B

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

H134R

Promoter

synapsin

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-oScarlet

Plasmid

#137137

Purpose

Intersectional viral expression of oScarlet in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183519

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Has Service

AAV8

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-sRGECO

Plasmid

#137127

Purpose

Intersectional viral expression of sRGECO in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-pBG-mCRE98-NLS-EGFP-WPRE

Plasmid

#239977

Purpose

adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

Depositor

Sinisa Hrvatin

Article

Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

EGFP (eGFP Synthetic)

Use

AAV

Promoter

pBG + mCRE98

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230990

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Jaws-KGC-GFP-ER2

Plasmid

#153537

Purpose

AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version).

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

EF1α1.1

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

Plasmid

#197066

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

CRISPR

Tags

FKBP and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

Plasmid

#170175

Purpose

Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

Plasmid

#158987

Purpose

Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP160

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(0.4)-Lyn-PHR(E281A)-HA

Plasmid

#180591

Purpose

Control for Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

PHR

Use

AAV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold

Plasmid

#226921

Purpose

Encoding SauriABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSauriCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

Plasmid

#75082

Purpose

An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Nuc-eYFP

Use

AAV and Cre/Lox

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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