We narrowed to 18,395 results for: tec

1. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFPL-Gg

   Plasmid

   #61653

   Purpose

   Gateway vector for generating green fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-scFvGCN4sfGFPTET1CD

   Plasmid

   #82561

   Purpose

   Expression vector for antibody-sfGFP-TET1CD.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   scFvGCN4sfGFPTET1CD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB4906

   Plasmid

   #106150

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDonor14-AcHELIX-KanR (CJT96)

   Plasmid

   #181794

   Purpose

   pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCBB-eGFP

   Plasmid

   #32548

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Consitutive lac

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. GW1-mScarlet-Epac-GeNL

    Plasmid

    #207874

    Purpose

    Mammalian expression of the FRET/BRET cAMP sensor

    Depositor

    Mathew Tantama

    Article

    French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

    Insert

    mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB6633

    Plasmid

    #106162

    Purpose

    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    unknown
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-G1(6/7FTrp)RS

    Plasmid

    #207621

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-G1(4/5FTrp)RS

    Plasmid

    #207620

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbS8c-ddcpf1-rfp

    Plasmid

    #153038

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYL-kasOp-FnCas12a2

    Plasmid

    #177874

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    kasOp*

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB4080

    Plasmid

    #215249

    Purpose

    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT277

    Plasmid

    #122608

    Purpose

    express evoCDA1-BE4max in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoCDA1-BE4max
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F23S A123V I195F

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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