We narrowed to 10,047 results for: yeast

1. PCUP1-4V5-Pho8-Met

   Plasmid

   #207029

   Purpose

   Expression of 4V5-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xV5

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BS-Trp-PCUP1-4myc-Pho8

   Plasmid

   #207028

   Purpose

   Expression of 4Myc-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCUP1-Vac8-GFP-Ura

   Plasmid

   #207027

   Purpose

   Expression of Vac8-GFP under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vac8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCUP1-GFP-Vma13-Ura

   Plasmid

   #207026

   Purpose

   Expression of GFP-Vma13 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vma13 ORF
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCUP1-mRuby3-Pho8-406

   Plasmid

   #207043

   Purpose

   Expression of mRuby3-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOA-Leu-FvPPT1

   Plasmid

   #179923

   Purpose

   Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Insert

   FvPPT1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Promoter

   Yarrowia lipolytica EXP1

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBT9-GGA2-GAE

   Plasmid

   #199175

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA2-GAE domain (GGA2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Contains Lys 545 Gln substitution

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBT9-GGA3-GAE

   Plasmid

   #199176

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   GGA3-GAE domain (GGA3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBT9-γ2 ear

   Plasmid

   #199178

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   AP-1 γ2 ear domain (AP1G2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBT9-GGA1-GAE

    Plasmid

    #199174

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    GGA1-GAE domain (GGA1 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACT2-μ1B (137-423)

    Plasmid

    #198172

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1B (137-423)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBT9-AAGAB

    Plasmid

    #198332

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AAGAB (AAGAB Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT174_GalL_A3A-Y130F-BE3

    Plasmid

    #145123

    Purpose

    Expressing base editor A3A-Y130F-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(Y130F); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT173_GalL_A3A-R128A-BE3

    Plasmid

    #145122

    Purpose

    Expressing base editor A3A-R128A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(R128A)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(R128A); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZ_P-HXT1-FAS1up

    Plasmid

    #126746

    Purpose

    vector for replacing the promoter of FAS1 coding gene

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    fas1 upstream
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    HXT1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTH394-SUP45-L34S

    Plasmid

    #29373

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L34S mutant gene (corresponding to the sup45-36ts…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only