We narrowed to 18,467 results for: Met

1. pET28a-PTBP1-RRM34

   Plasmid

   #89156

   Purpose

   Recombinant expression of PTBP1-RRM34 in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   contains RNA Recognition Motif 3+4

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB2-mRuby3 Addgene

   Plasmid

   #104005

   Purpose

   Plasmid encoding mRuby3 Addgene

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRuby3 Addgene
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

   Plasmid

   #197893

   Purpose

   Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   mCherry
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   EF1a

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCardinal-H2B-C-10

   Plasmid

   #56162

   Purpose

   Localization: Nucleus/Histones, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pHS18 (mANGPTL3-Strep)

   Plasmid

   #109111

   Purpose

   Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL3 (Angptl3 Mouse)
   

   Tags

   StrepII

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-VTKD1

   Plasmid

   #170846

   Purpose

   AAV backbone for transgene expression in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dronpa-DEVD-TagRFPT

   Plasmid

   #87704

   Purpose

   Fluorescent biosensor for super-resolution imaging of caspase activity.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   Dronpa-DEVD-TagRFPT
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCardinal-Tubulin-C-18

   Plasmid

   #56176

   Purpose

   Localization: Microtubules, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pLentiCRISPR-v1-sgGLS_2

   Plasmid

   #163459

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB2-mRuby3

    Plasmid

    #104004

    Purpose

    Plasmid encoding mRuby3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mRuby3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMG81-MBP-t-M.MpeI-N374K-His

    Plasmid

    #197985

    Purpose

    Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

    Depositor

    Rahul Kohli

    Article

    Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

    Insert

    MBP-t-M.MpeI-N374K-His
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    M.MpeI-N374K

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTYB11-PTBP1-RRM1

    Plasmid

    #89153

    Purpose

    Recombinant expression of PTBP1-RRM1 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)
    

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1_VNp-hGH

    Plasmid

    #182411

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-hGH (GH1 Human)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH_TNF-SBP-mCherry

    Plasmid

    #65283

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCardinal-PDHA1-N-10

    Plasmid

    #56172

    Purpose

    Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    PDHA1 (PDHA1 Human)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pSCALPS-mAgo2-EGFP

    Plasmid

    #209935

    Purpose

    Lentiviral expression of mouse Argonaute-2 in mammalian cells

    Depositor

    Phillip Zamore

    Article

    Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-VTKS3

    Plasmid

    #170854

    Purpose

    AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVPT-GDNF-rtTR-KRAB-2SM2

    Plasmid

    #11647

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for GDNF (mPGK promoter) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off (GDNF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pECE HA AMPKa1 AS

    Plasmid

    #69831

    Purpose

    Expresses Expresses AMPK alpha 1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-mCherry-Zdk1-RhoA Q63L

    Plasmid

    #81058

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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