We narrowed to 4,396 results for: erf

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pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

Plasmid

#71219

Purpose

dCas9 fused to the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Lentiviral

Tags

dSpCas9-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

Plasmid

#188770

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Islr2.b-Fc-His

Plasmid

#72077

Purpose

Expresses the extracellular region of the Islr2.b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Islr2.b-AP-His

Plasmid

#71951

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr2.b (Islr2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.1-Fc-His

Plasmid

#72081

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP6 57-98

Plasmid

#242420

Purpose

Expression of the second alpha helix of CHMP6 (residues 57-98), N-terminally tagged with sfGFP

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP6 (CHMP6 Human)

Tags

sfGFP (superfolder GFP)

Expression

Mammalian

Mutation

Residues 57-98

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT-sm_PCaspase

Plasmid

#214048

Purpose

Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT-sm

Plasmid

#214033

Purpose

Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Tags

Strep-tag II

Expression

Bacterial

Mutation

R456E, D459K, D476K, R479E

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins1

Plasmid

#195038

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO GLTP

Plasmid

#170732

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

GLTP (GLTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

Plasmid

#166814

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

Plasmid

#166812

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H1276G

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

Plasmid

#166813

Purpose

Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K1277E

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry E1461R

Plasmid

#164002

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry W1460A

Plasmid

#164001

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

W1460A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry M1457A

Plasmid

#164000

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

M1457A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry Y1445A

Plasmid

#163999

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD (dimer interface mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Y1445A

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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